双环蘑菇与双孢蘑菇多酚氧化酶活性的比较  

Comparison of activities of polyphenol oxidases between Agaricus bitorquis and A.bisporus strains

作　　者：蔡为明[1] 金群力[1] 杜新法[2] 方菊莲[1] 冯伟林[1] 范丽军[1] 

机构地区：[1]浙江省农业科学院园艺研究所,浙江杭州310021 [2]浙江省农业科学院植物保护与微生物研究所,浙江杭州310021

出　　处：《浙江农业学报》2003年第6期341-344,共4页Acta Agriculturae Zhejiangensis

摘　　要：分析测定6个双孢蘑菇菌株和4个双环蘑菇菌株子实体组织及子实体不同部位组织内的多酚氧化酶(PPO)活性表明,双环蘑菇子实体组织内的PPO活性明显比双孢蘑菇菌株的低。双环蘑菇菌株酶液的OD436值在0.211～0.245之间,而双孢蘑菇菌株酶液的OD436值大于0.273。由此推测,双环蘑菇抗褐变性与其子实体组织内的PPO活性低有关。双环蘑菇子实体不同部位组织内的PPO活性差异不明显。而双孢蘑菇子实体不同部位组织内的PPO活性存在差异。菌株4607,3003,2796菇表层组织及菌褶和近菌褶组织PPO活性显著高于菌肉和菌柄组织,菌株4580,176,U3菇表层组织及菌褶和近菌褶组织PPO活性略高于菌肉和菌柄组织。Activities of polyphenol oxidases (PPO) in the tissues of different position of organism on fruitbody for 6 strains of Agaricus bisporus and 4 strains of Agaricus bitorquis were analyzed. The results indicated that PPO activities in the tissues of fruitbodies of A. bitorquis strains at growing stage were markedly lower than that of A. bisporus strains. The OD436 values in the enzyme liquids of A. bitorquis strains were 0.211-0.245 and the ones of A. bisporus strains were higher than 0.273. It was suggested that the antibrowning capacity of A.bitorquis was related to the lower activities of polyphenol oxidases in the fruitbodies.The PPO activities in different ppositions of organisms on the fruitbodies for A. bitorquis strains were similar and the ones for A. bisporus strains were different.

关 键 词：双环蘑菇 双孢蘑菇 多酚氧化酶 蘑菇褐变 

分 类 号：S646.11[农业科学—蔬菜学]