DYSFUNCTION OF MYOCARDIAL AND VASCULAR TAURINE TRANSPORT IN L-N~ω-NITRO-ARGININE-INDUCED HYPERTENSIVE RATS  

作　　者：石彦荣 齐永芬 卜定方 蒋宏峰 王冬艳 高霖 庞永正 唐朝枢 

机构地区：[1]Institute of Cardiovascular Disease Research,Peking University First Hospital,Beijing100034Institute of Cardiovascular Research,Peking University Health Science Center,Beijing100083 [2]Institute of Cardiovascular Disease Research,Peking University First Hospital,Beijing100034 [3]Institute of Cardiovascular Research,Peking University Health Science Center,Beijing100083

出　　处：《Chinese Medical Sciences Journal》2003年第4期199-205,共7页中国医学科学杂志（英文版）

基　　金：ThisworkwassupportedbyTheMajorBasicResearchPro-gramofChina(G200056905).

摘　　要：Objective.To study the change of taurine transport,and taurine transporter(TAUT)and cysteine sulfinate decarboxylase(CSD)mRNA contents in hypertension and hypertensive cardiomegaly.Methods.Taurine content was determined with a amino acid analyser.Taurine uptakes were deter-mined by( 3 H)-taurine incubation.The content of TAUT and CSD mRNA levels were measured by com-petitive quantitative RT-PCR in myocardial and vascular tissues of L-N ω -nitro-arginine(L-NNA)-in-duced hypertensive rats.Results.After the treatment of rats with L-NNA for28days,myocardial and vascular taurine con-tents decreased by11%and15%(P<0.05),respectively,and plasma taurine level increased by13%(P<0.05).Myocardial and vascular Vmax of taurine uptake reduced by30%and19%(P<0.05),respec-tively.Their Km of taurine uptake increased by36%and17%(P<0.05).Myocardial and vascular TAUT mRNA content decreased by22%and19%(P<0.05),respectively,but CSD mRNA content increased by22%(P<0.05)and30%(P<0.01),respectively.Conclusions.This study suggests that there is a decreased taurine content in myocardial and vascu-lar tissues of L-NNA-induced hypertension rats.The decreased taurine content may result from the de-creased number of TAUT on the cell membrane mainly due to the down-regulation of TAUT gene and TAUT affinity caused by hypertension and hypertensive cardiomegaly.

关 键 词：L-N    ω    -nitro-arginine taurine transporter cysteine sulfinate decarboxylase 

分 类 号：R541.3[医药卫生—心血管疾病]