猪白细胞Ⅱ类抗原基因多态性研究进展  

Advances in Allelic Polymorphism at Class Ⅱ of the Pig MHC(SLA)

作　　者：周波[1] 王林云[1] 谈永松[2] 张似青[2] 

机构地区：[1]南京农业大学动物科技学院,南京210095 [2]上海市农业科学院畜牧兽医研究所,上海201106

出　　处：《遗传》2003年第5期611-614,共4页Hereditas（Beijing)

基　　金：高等学校博士学科点专项科研基金(编号:1999030704)资助项目

摘　　要：本文对SLAⅡ类基因的染色体图谱定位、分子结构、基因分型及其多态性的研究进展作了介绍。重点介绍了SLAⅡ类基因的RFLP基因分型及其多态性研究。猪的主要组织相容性复合物Ⅱ类抗原基因座位存在不同程度的等位基因多态性。RFLP技术是一种简便而快捷的SLAⅡ类等位基因分型方法。SLAⅡ类基因的多态性和抗原肽结合位点密切相关,这些位点能结合处理过的抗原,然后递呈给T细胞。另外,在编码β1链的SLA DRB1基因和SLA DQB基因中有4个富含GC序列区。This article gave a detailed introduction about regional map,molecular structure,genotyping and polymorphism of SLA class Ⅱ genes.The pig major histocompatibility complex (MHC) class Ⅱ antigens have been known to exhibit a different degree of allelic polymorphism.The locus specific oligonucleotide primers and RFLP analysis provide a simple and rapid method for genotyping expressed SLA class Ⅱ from genomic DNA.SLA class Ⅱ polymorphism was related to the antigenic peptide binding sites.Detailed analysis of sequences showed that there were 4 GC rich sequences in exon 2 of SLA DQB and SLA DRB1 genes.

关 键 词：猪 白细胞Ⅱ类抗原基因 多态性 RFLP 

分 类 号：S828[农业科学—畜牧学]