Ⅰ型人免疫缺陷病毒gag-gp120嵌合基因核酸疫苗的构建与鉴定  

Construction and Identification of DNA vaccine containing chimeric gene gag-gp120 of HIV-1

作　　者：江文正[1] 金宁一[1] 韩文瑜[1] 

机构地区：[1]解放军军需大学全军基因工程重点实验室,吉林长春130062

出　　处：《细胞与分子免疫学杂志》2003年第4期341-342,345,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家杰出青年基金资助项目 (No .3982 51 1 9)

摘　　要：目的 :构建含Ⅰ型人免疫缺陷病毒 (HIV 1) gag gp12 0嵌合基因核酸疫苗的表达质粒。方法 :将gag和 gp12 0连接后的嵌合基因插入到真核表达载体 pVAX1中 ,构建真核表达质粒pVAXGE。用脂质体法将构建的重组质粒转染Hela细胞 72h后 ,取转染的Hela细胞进行RT PCR检测和和Dot ELISA分析。结果 :重组质粒转染细胞的总RNA中 ,可扩增出目的基因的转录产物。Dot ELISA的结果显示 ,目的基因在Hela细胞内得到表达。结论 :成功地构建了表达gag gp12 0嵌合基因的核酸疫苗质粒 ,为HIVAIM: To construct DNA vaccine expresseion plasmid containing the chimeric gene gag gp120 of HIV 1. METHODS: The recombinant eukaryotic expression vector pVAXGE was constructed via inserting the chimeric gene gag gp120 into the vector pVAX1. Hela cells had been transfected by recombinant plasmid via liposome. After 72 h, the transfected cells was detected by RT PCR and analyzed by Dot ELISA. RESULTS: The transcript products of target gene could be amplified from the cells transfected by recombinant plasmid. Dot ELISA detection showed that the target gene was expressed in Hela cells. CONCLUSION: The DNA vaccine plasmid expressing chimeric gene gag gp120 is successfully constructed, which lays the foundation for preparing DNA vaccine against HIV 1.

关 键 词：Ⅰ型人免疫缺陷病毒 gag-gp120 嵌合基因 核酸疫苗 

分 类 号：R373.9[医药卫生—病原生物学]