从噬菌体环肽库中筛选TNF-α表位模拟肽的研究  

Screening of TNF-α mimotopes from c7c phage display peptide library

作　　者：罗海波[1] 刘北一[1] 刘艳君[1] 朱平[1] 富宁[1] 

机构地区：[1]第一军医大学免疫学教研室,广东广州510515

出　　处：《细胞与分子免疫学杂志》2003年第4期322-325,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金资助项目 (No .3980 0 1 2 6)

摘　　要：目的 :用具有中和活性的抗TNF α单抗 (mAb)J1D9筛选噬菌体环七肽库 ,从中获得可模拟TNF α表位的短肽。方法 :筛选TNF α表位模拟肽 ,并用夹心ELISA法鉴定阳性克隆。结果 :对环七肽库进行 3轮筛选后 ,随机挑选 2 0个噬菌体克隆 ,经ELISA鉴定出 9个阳性克隆。DNA序列分析后 ,推导的氨基酸序列共 3种 :c RRPAQSG c、c NKHNRKI c和c RGMSRKI c。结论 :筛选的环七肽克隆展示肽具有TNF α的抗原性 ,为TNFAIM: To acquire oligo peptides mimicking TNF α epitopes. METHODS: The TNF α mimotopes were screened from c7c phage display peptide library by using neutraliz ing mAb J1D9 against TNF α, and positive clones were identified by sandwich ELISA. RESULTS: 9 out of 20 phage clones were identified as positive clones which can bind to mAb J1D9. The dedu ced amino acid sequence analysis showed three different sequences: c RRPAQSG c, c NKHNR KI c and c RGMSRKI c. CONCLUSION: All of the three peptides obtained can mi mic the epitopes of TNF α and bind to mAb J1D9.

关 键 词：噬菌体肽库 TNF—α 表位模拟肽 

分 类 号：R392.33[医药卫生—免疫学]