青藤碱对小鼠吗啡的精神依赖以及cAMP水平的影响  

Effects of sinomenine on the psychic dependence on morphine and the brain cyclic AMP level in mice

作　　者：王彩云[1] 莫志贤[1] 邵红霞[1] 

机构地区：[1]第一军医大学中医系药理药化学教研室,广州510515

出　　处：《中国药理学通报》2003年第5期575-577,共3页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助课题;No 3 0 2 71610

摘　　要：目的　观察中药单体成分青藤碱 (sinomenine ,Sin)对吗啡诱导的小鼠精神依赖及其中枢cAMP水平的变化的影响。方法　采用有倾向性程序引起小鼠显著的位置偏爱效应 ,利用条件性位置偏爱实验观察ipSin 10、30、6 0mg·kg-1对小鼠吗啡奖赏效应的影响 ,用放射免疫法测定小鼠脑中cAMP含量。结果　Sin可剂量依赖性地缩短小鼠在吗啡伴药箱的时间 ,对吗啡慢性作用引起的小鼠脑内cAMP含量增加具有抑制作用。结论　Sin能减弱小鼠对吗啡的精神依赖 ,降低中枢cAMP水平 ,且其本身未显示精神依赖性反应。AIM To explore the effects of sinomenine (Sin) on psychic dependence induced by morphine (Mor) in mice and its relation to the cAMP content of the cerebral cortex. METHODS A biased procedure induced the strong place preference. The effect of Sin (10, 30, 60 mg·kg -1 , ip) on the rewarding property of morphine was observed in a conditioned place preference(CPP) paradigm in mice,at the same time, the change of cAMP content in cerebral cortex was measured by the radioimmunoassay(RIA). RESULTS Sin significantly shortened the time of staying in morphine-pair compartment and lowered the cAMP level of mice. CONCLUSION Sin could attenuate effects of psychical dependence induced by Mor, its mechanism may be related to decrease of cAMP level in the brain, and Sin did not display psychical dependence in the experiment.

关 键 词：小鼠 青藤碱 吗啡 条件性位置偏爱 CAMP 精神 依赖 

分 类 号：R284.1[医药卫生—中药学] R971.2[医药卫生—中医学]