应用脉冲凝胶电泳技术分析肺炎克雷伯菌染色体多态性  

Analysing genomic polymorphysm of Klebsiella pneumoniae by pulsed-field gel electrophoresis

作　　者：叶杨芹[1] 马佳毓[1] 张文莉[1] 付英梅[1] 赵月辉[1] 

机构地区：[1]哈尔滨医科大学微生物教研室,150086

出　　处：《中华微生物学和免疫学杂志》2003年第4期314-316,共3页Chinese Journal of Microbiology and Immunology

基　　金：国家自然科学基金资助项目 ( 30 170 816 )

摘　　要：目的　利用脉冲凝胶电泳 (PFGE)技术探讨肺炎克雷伯菌 3个亚种临床分离株核型特征。方法　用XbaⅠ酶切各试验菌株染色体DNA ,经脉冲凝胶电泳分离酶切片段。结果　PFGE法将2 8株细菌分为 15个型和亚型 ,较准确地显示各菌株染色体多态性 ,并筛选出亚种间的差异DNA片段。结论　脉冲凝胶电泳技术分析肺炎克雷伯菌基因型准确、可靠 ,是用于流行病学监测的有效方法。Objective To analyse the genomic polymorphysm of three subspecies of Klebsiella pneumoniae by pulsed-field gel electrophoresis (PFGE). Methods The genomic DNA was digested with 40U of XbaⅠ restriction endonuclease and then PFGE was carried out with CHEF-Ⅲ system. Results A total of 15 types and subtypes were identified by PFGE which showed DNA polymorphism of this species and specific fragments of subspecies were selected. Conclusion PFGE is an accurate and liable typing method to analyze Klebsiella pneumoniae. It can be used as epidemiological surveillance. [

关 键 词：脉冲凝胶电泳技术 肺炎克雷伯菌 染色体 多态性 流行病学 监测 

分 类 号：R378[医药卫生—病原生物学]