曲马多加强针刺对关节炎大鼠的镇痛作用  

Analgesic Effect of Acupuncture Enhanced by Tramadol in Arthritic Rats

作　　者：谢虹[1] 马飞[1] 高秀[1] 吴根诚[1] 

机构地区：[1]复旦大学上海医学院针刺原理研究所

出　　处：《针刺研究》2003年第1期38-41,共4页Acupuncture Research

摘　　要：目的 :观察不同剂量曲马多对关节炎大鼠热痛敏的镇痛作用及曲马多对针刺镇痛的影响。方法 :在完全弗氏佐剂关节炎大鼠模型上采用辐射热缩腿反射的方法 ,以痛敏分数作为观察指标 ,分别腹腔注射 (i.p .) 5mg·kg- 1 ·d- 1 、1 0mg·kg- 1 ·d- 1 、2 0mg·kg- 1 ·d- 1 曲马多和 1 0mL·kg- 1 ·d- 1 生理盐水 1 0天 ,记录每次给药 2 4hr后痛敏分数变化。继而选择 1 0mg·kg- 1 ·d- 1 曲马多与电针合用 ,观察单独应用 1 0mg·kg- 1 ·d- 1 曲马多、单独应用电针、曲马多与电针合用及1 0mL·kg- 1 ·d- 1 生理盐水四组 (均为 1 0天 ) ,每次给药或 /和电针 2 4hr后痛敏分数变化。结果 :i.p .5mg·kg- 1 ·d- 1 曲马多连续应用 1 0天时 ,大鼠痛敏分数无明显变化 ;i.p.1 0mg·kg- 1 ·d- 1 曲马多 9天时大鼠痛敏分数显著提高 ;i.p .2 0mg·kg- 1 ·d- 1 曲马多 6天时大鼠的痛敏分数显著提高 ;单独应用电针 8天时大鼠痛敏分数显著提高 ;i.p.1 0mg·kg- 1 ·d- 1 曲马多与电针合用 4天时可明显提高大鼠痛敏分数。结论 :长期应用曲马多对慢性炎症痛大鼠具有镇痛作用 ,呈剂量相关性 ;曲马多与电针合用可加强电针的镇痛作用 ,并降低曲马多使用剂量。Objective:To investigate the antinociceptive effects of chronically using different dose of tramadol and the effect of tramadol on acupuncture analgesia in arthritis rats. Methods: SD rat arthritis model was established by injection of complete Freund's adjuvant 0.05 mL into the left ankle joint. The paw withdrawal latency (PWA)was detected with a radiant heat algesimeter. The difference between PWA of the inflammatory side and the healthy side was used as the hyperalgesia score. By using paw withdrawal latency to radiant heat stimulation and hyperalgesia score as the endpoint of nociceptive test, 5 mg·kg -1 ·d -1 , 10 mg·kg -1 ·d -1 and 20 mg·kg -1 ·d -1 tramadol and 1 mL·kg -1 ·d -1 0.9% normal saline (NS) were respectively administrated intraperitoneally, once everyday and continuously for ten days. The changes of hyperalgesia score were observed 24 hr after drug administration everyday following i.p. 10 mg ·kg -1 ·d -1 tramadol, electroacupuncture (EA), EA combined with i.p.10 mg·kg -1 ·d -1 tramadol and i.p. 1 mL·kg -1 ·d -1 0.9% NS (4 groups), once everyday for ten days. Results: I.p. 5 mg·kg -1 ·d -1 tramadol for ten days did not show any effect ( P > 0.05) . I.p.10 mg·kg -1 ·d -1 tramadol for nine days, i.p. 20 mg·kg -1 ·d -1 for six days and using EA for eight days elevated hyperalgesia score significantly in comparison with NS group ( P <0.05～ 0.01) . Then i.p.10 mg·kg -1 ·d -1 tramadol combined with electroacupuncture for four days, hyperalgesia score was elevated significantly in comparison with NS group ( P <0.05～0.01) and with EA group ( P <0.05). Conclusion: The results show that tramadol has dose relative antinociceptive effect,and tramadol can enhance the analgesic effect of acupuncture under lower dose, suggesting that combination of acupuncture with tramadol is one of the effective treatment methods for chronic pain.

关 键 词：曲马多 针刺 关节炎 镇痛作用 痛觉调制 

分 类 号：R246.2[医药卫生—针灸推拿学]