褪黑素和电针对创伤大鼠淋巴细胞转化功能、IL-2活性及ACTH水平的影响  

Effects of Melatonin and Electroacupuncture on Lymphocyte Proliferation,IL-2 Activity and ACTH Levels in Traumatic Rats

作　　者：黄颖苏[1] 姜建伟[1] 吴根诚[1] 曹小定[1] 

机构地区：[1]复旦大学医学院神经生物学教研室

出　　处：《针刺研究》2003年第1期42-47,共6页Acupuncture Research

基　　金："九.五"攻关计划 (No 96 90 6 11 0 1)基金资助

摘　　要：目的 :观察褪黑素和电针对创伤大鼠脾脏淋巴细胞转化功能、ConA诱导的IL 2活性及脾淋巴细胞、血浆、下丘脑及垂体ACTH含量的影响。方法 :MTT法检测淋巴细胞转化功能、IL 2活性 ,放射免疫分析法检测各组织ACTH的含量。结果 :腹腔注射褪黑素能有效逆转手术创伤大鼠的淋巴细胞转化功能 ,低水平IL 2诱生活性得到恢复 ,同时创伤后升高的脾淋巴细胞、血浆、下丘脑ACTH水平下降 ,而垂体内降低的ACTH水平回升。电针刺激“足三里”、“阑尾”穴也能明显改善创伤应激诱导的免疫抑制 ,并降低脾淋巴细胞、血浆、下丘脑ACTH含量 ,增加垂体ACTH含量。褪黑素与电针合用可进一步调节被抑制的免疫功能 ,且与单独褪黑素或电针相比有显著差异。结论 :褪黑素和 /或电针能恢复创伤大鼠降低的淋巴细胞转化功能、IL 2诱生活性 。Objective: To observe the effect of melatonin (MT) and electroacupuncture (EA) on the spleen lymphocyte proliferation, concanavaline (Con) A induced IL 2 activity and ACTH content in the spleen lymphocyte supernate, plasma, hypothalamus and the pituitary of traumatic rats. Methods: SD rats with body weight of 200±20 g were randomly divided into normal control group, traumatic injury (TI) +MT solvent control group, TI+EA group, TI+MT group and TI+MT+EA group, with 6 cases in each group. Following anesthesia with pentobarbital sodium (0.4%, 2 mL), a longitudinal incision was made along the back and the abdominal midline to mimic clinical visceral exploring surgical operation for establishing traumatic injury model. EA (2 Hz, 1 mA) was applied to 'Zusanli'(ST 36) and 'Lanwei'(Extra 37) for 60 min before intraperitoneal (i.p.) injection of MT (5%, 5 mg/kg) that was given at 4～5 o'clock of the operation day. MTT colorimetric assay was used to detect lymphocyte proliferation rate and Con A induced IL 2 activity of the spleen lymphocyte supernate, and radioimmunoassay was used to detect ACTH contents in the spleen lymphocytes, plasma, hypothalamus and the pituitary. Results: Administration of MT and EA were able to revise TI generated depression of the lymphocyte proliferation rate and IL 2 activity ( P <0.05) compared with TI+MT solvent control group. In comparison with MT+TI and EA+TI groups, both the lymphocyte proliferation rate and IL 2 activity were raised further in TI+MT+EA group ( P <0.05). Intraperitoneal administration of MT and EA could considerably lower traumatic injury induced increase of ACTH levels in the spleen lymphocytes ( P <0.001), plasma ( P <0.05) and hypothalamus ( P <0.05), and significantly inhibit TI induced decrease of ACTH in the pituitary ( P <0.05, vs TI group respectively). When MT and EA used together, TI induced increase of ACTH levels in the spleen lymphocytes, plasma and the hypothalamus was lowered further ( P <0.05, vs TI+EA or MT group), while the de

关 键 词：褪黑素 电针 创伤 淋巴细胞 转化功能 IL-2 活性 ACTH 

分 类 号：R245.97[医药卫生—针灸推拿学]