兔尿中敌鼠及其代谢物的HPLC-DAD分析  

Study of diphacinone and its metabolites in rabbit urine by HPLC-DAD

作　　者：杨士云[1] 潘冠民[1] 孟广范[1] 张大明[1] 

机构地区：[1]北京市公安局法医检验鉴定中心,北京100085

出　　处：《中国法医学杂志》2003年第1期27-28,30,共3页Chinese Journal of Forensic Medicine

基　　金：北京市公安局科研项目资助

摘　　要：目的 尿中敌鼠及其代谢物的HPLC分析方法研究。方法 用CN柱和SAX柱的固相萃取(SPE)技术分离提取兔尿中的敌鼠及其代谢物,用香豆素作内标,用HPLC-DAD方法进行fenxi。色谱柱:Hypersil BDSC_(18)(150×4.6mm):保护柱:Phenomenex C_(18) (ODS,4×3.0mm,Octadecyl;流动相A:0.5％离子对A水溶液,B:0.5%离子对A甲醇液,梯度程序洗脱;DAD检测波长为311nm。结果 在中毒的兔尿中检出11种敌鼠代谢物。结论 此方法简单、准确、快速。Objective An analytical approach has been studied lor high performance liquid chro-matographic determination of Diphacinone and its metabolites extracted from urine. Method Diphacinone and its metabolites extracted from rabbit urine by solid phase extraction (SPE) cartridges (CN and SAX) were analyzed with coumarin as the internal standard. Diphacinone and its metabolites were separated by reversed-phase gradient chromatography with DAD detector at 311nm. The analytical column was Hypersil BDS C18 (150mm×4.6nmi. d. ) and the guard column was Phenomenex ODS (4mm×3.0mmi. d.). The mobile phase was a gradient mixture of aqueous solution (A) and methanol solution (B), both containing 0.5% ion pair A. Results Eleven diphacinone metabolites were detected in poisoned rabbit urine by this method. Conclusion This method is simple, exact and rapid.

关 键 词：尿 敌鼠 代谢物 HPLC-DAD分析 

分 类 号：D919[医药卫生—法医学]