烟蒂DNA分型的研究  

Study on DNA extracted from cigarette butts

作　　者：张庆霞[1] 刘雅诚[1] 唐晖[1] 王静[1] 严江伟[1] 高俊薇[1] 

机构地区：[1]北京市公安局法医检验鉴定中心,北京100085

出　　处：《中国法医学杂志》2003年第1期29-30,共2页Chinese Journal of Forensic Medicine

基　　金：公安部科研项目(编号 20011121401)

摘　　要：目的 研究烟蒂中DNA提取及其检验。方法 用Chelex-100法提取170枚烟蒂样本的DNA,进行PCR扩增及STR检验。结果 除1名志愿者提供的21枚烟蒂外层纸未能检出STR基因分型外,其余烟蒂外层纸均得到分型结果。加入少许烟丝的样本未能检出STR分型,与口唇接触的海绵有时可检出基因型,6个月内的烟蒂可检出小片段基因座。结论 烟蒂能进行DNA分型,在法医检案中具有应用价值。Objective To study the DNA extraction and its typing in cigarette butts. Method Chelex 100 extraction method was used to extract DNA of 170 cigarette butts, STR loci were typed after PCR amplification by Profiler Plus kit. Results 21 cigarette butts from one volunteer have no results, the others were all obtained typing results. The samples which were added a little tobacco have no results. Sometimes DNA in sponge contacted with mouth was typed. The small fragment loci were typed in cigarette butts which had been reserved for six months. Conclusion Cigarette butt can be typed by DNA analysis, which is useful in forensic identification.

关 键 词：烟蒂 DNA分型 PCR 法医检验 

分 类 号：D919[医药卫生—法医学]