甘薯和近缘野生种Ipomoea triloba的种间体细胞杂种植株再生  

Regeneration of Interspecific Somatic Hybrid between Sweetpotato and Its Wild Relative Ipomoea triloba

作　　者：王晶珊[1] 刘庆昌[2] 孟祥霞[1] 王维华[1] 徐丽娟[1] 翟红[2] 

机构地区：[1]莱阳农学院农学系,山东莱阳265200 [2]中国农业大学植物遗传育种系,北京100094

出　　处：《农业生物技术学报》2003年第1期40-43,共4页Journal of Agricultural Biotechnology

基　　金：山东省教委博士点基金;国家863(2001AA241181)资助项目。

摘　　要：将甘薯(Ipomoeabatatas )品种元气和近缘野生种I.triloba的叶柄原生质体用PEG法融合后,培养在含有0.05～0.20mg/L2,4-D和0.5mg/LKT的改良MS液体培养基中。培养约7周后,将直径达1～2mm的小愈伤组织转移到添加0.05～0.20mg/L2,4-D和0～0.5mg/LKT的MS培养基上使其增殖。转移3～5周后,愈伤组织直径达8～15mm。将这些愈伤组织再转移到MS基本培养基上,或转移到添加3.0mg/LBAP的MS培养基上培养4～5周后再进一步转移到MS基本培养基上进行培养,结果获得了69株再生植株。RAPD分析表明,其中1株再生植株是元气和I.triloba的种间体细胞杂种。杂种植株的株型呈野生攀缘型,相似I.triloba;而茎色呈绿-紫色,相似元气;其茎的节间长度和叶片形状表现为融合双亲的中间型。茎尖细胞染色体数为54～103,因细胞不同而不同。Protoplasts of sweetpotato (Ipomoea batatas ) cv. Genki were fused with protoplasts of its wild relative I. triloba by the PEG method. The fusion products were cultured in a modified liquid MS medium containing 0.05～0.20 mg/L 2,4-D and 0.5 mg/L KT. After 7 weeks of culture, small calluses 1～2 mm in size were transferred to solid MS medium supplemented with 0.05～0.20 mg/L 2,4-D and 0～0.5 mg/L KT for callus proliferation. When the calluses were transferred either to the basal medium or to the medium supplemented with 3.0 mg/L BAP and further to the basal medium, 69 plants were regenerated. PCR analysis indicated that 1 of 69 regenerated plants was the somatic hybrid. This somatic hybrid showed the climbing habit looked like I. triloba and the stem color, green to purple, looked like Genki. The internode length and the leaf shape were intermediate. The chromosome number of root tip cells ranged from 54 to 103, depending on different root tip cells.

关 键 词：甘薯 近缘野生种 体细胞 杂种 植株再生 

分 类 号：S531[农业科学—作物学]