用cDNA芯片检测大鼠心肌缺血预适应后基因表达谱的改变  

The Changes of Gene Expression Profiles after Myocardical Ischemic Preconditioning in Rats

作　　者：吕青兰[1] 袁灿[1] 张华莉[1] 陈广文[1] 王尧玲[1] 邓恭华[1] 涂自智[1] 肖献忠[1] 

机构地区：[1]中南大学湘雅医学院病理生理学教研室,湖南省长沙市410078

出　　处：《中国动脉硬化杂志》2003年第3期189-193,共5页Chinese Journal of Arteriosclerosis

基　　金：国家自然科学基金 ( 3 0 170 3 733 0 2 70 5 3 3 );国家 973重点项目 (G2 0 0 0 0 5 690 8)资助

摘　　要：为了检测缺血预适应后大鼠心肌基因表达谱的改变 ,采用cDNA芯片方法对缺血预适应后大鼠心肌基因谱的改变进行检测。结果发现 ,缺血预适应大鼠心肌中差异表达基因共 75项 ,其中表达上调基因 4 8项 ,表达下调基因 2 7项。采用逆转录—聚合酶链反应进一步验证了cDNA芯片结果的可靠性 ,并初步分析了基因表达谱改变在缺血预适应诱导的心肌内源性保护作用中有一定意义。以上研究有助于从基因水平阐明缺血预适应诱导的心肌保护的分子机制 ,为临床防治缺血 再灌注损伤及缺血性心脏疾病提供新的思路。Aim To test the changes of gene expression profiles after myocardical ischemic preconditioning in rats. Methods The changes of gene expression profiles of 4 096 genes in rat hearts were observed using cDNA chip and the significance of the changes in gene expression was analyzed. Results The expression of 48 genes was up regulated and 27 genes was down regulated after ischemic preconditioning. The changes in gene expression were further confirmed by RT PCR. The significance of the changes of gene expression profiles in the myocardial protection induced by ischemic preconditioning was analyzed. Conclusions Ischemic preconditioning resulted in the changes of myocardical gene expression profiles. The results were helpful to elucidate the molecular mechanism of myocardial protection induced by ischemic preconditioning and provided new clues for prevention and treatment of myocardial ischemia reperfusion injury and ischemic heart diseases.

关 键 词：病理生理学 心肌缺血 基因表达谱 缺血预适应 大鼠 CDNA芯片 差异表达 

分 类 号：R542.2[医药卫生—心血管疾病]