金葡液对冻干异体骨移植愈合影响的实验研究  

Experimental Study of Jinpuye on the Healing of Freeze-Dried Bone Allograft

作　　者：何钢[1] 顾洁夫[1] 徐振华[1] 黄师[1] 

机构地区：[1]武汉大学第二临床学院骨科,武汉430071

出　　处：《医学新知》2003年第4期210-212,共3页New Medicine

摘　　要：目的　观察金葡液对冻干异体骨移植愈合的影响 ,并探讨其作用机制。方法　 2 4只兔右侧桡骨造成 15mm缺损模型 ,植入冻干异体骨 ,随机分成两组 ,每组 12只。术后第 10d开始 ,实验组局部注射金葡液 ,对照组注射等体积的生理盐水。术后不同时间分别行X线片、组织学和放射性核素骨扫描检查 ,并测定血清钙、磷及碱性磷酸酶(ALP)含量。结果　实验组术后不同时间的骨代谢活性、新骨生成和血清P及ALP含量均高于对照组 ,而血清Ca低于对照组。实验组异体骨 90d完全愈合 ,对照组仍未愈合。结论　金葡液作为一种促进骨折愈合药物 ,可有效加快冻干异体骨的愈合 ,加速新骨生成。Objective To study the effect of Jinpuye on the healing of freeze-dried bone allograft and explore its mechanisms. Methods The 15mm segmental bone/periosteum defects were created on right radius in 24 New Zealand rabbits,filled with freeze-dried bone allograft, and were divided into two groups randomly, 12 in each group. Ten days after operation, experimental group were injected with Jinpuye locally, control group were injected with saline. Observations were made by X-ray, radionuclide bone imaging, histological examination as well as measurement of calcium, phosphorus and alkaline phosphatase(ALP) in serum at different periods postoperatively. Results The new bone formation, bone metabolic activity, amount of phosphorus and ALP in serum were higher in experimental group than in that of control, while the level of serum calcium was on the contrary. The freeze dried-bone allograft healed on the 90th day in experimental group, but did not heal at the same time in control group. Conclusions As a fracture healing medicine, Jinpuye promotes the new bone formation, accelerates the healing of freeze-dried bone allograft effectively.

关 键 词：金葡液 冻干异体骨移植 作用机制 骨缺损 动物模型 骨折愈合 组织形态学 

分 类 号：R687.3[医药卫生—骨科学]