一氧化氮合酶抑制剂L-精氨酸对创伤性休克大鼠存活率的影响  

Influence of inhibitors of nitric oxide synthase and L-arginine on the survival rate in a rat model of traumatic shock

作　　者：黄宗海[1] 冯浩淼[1] 黄绪亮 孙英刚[1] 林洪武[1] 

机构地区：[1]第一军医大学珠江医院普外科,广州510282 [2]全军休克微循环重点实验室

出　　处：《中国危重病急救医学》2004年第1期45-48,共4页Chinese Critical Care Medicine

基　　金：广东省自然科学基金资助项目 (0 0 10 48)

摘　　要：目的　探讨一氧化氮合酶 (NOS)抑制剂左旋精氨酸 (L Arg)对创伤性休克大鼠存活率的影响。方法　通过外力致大鼠双侧股骨骨折 ,建立创伤性休克模型。复苏时分别给予非选择性 NOS抑制剂 L 硝基精氨酸甲酯 (L NAME) 10 m g/kg、选择性诱导型 NOS(i NOS)抑制剂氨基胍 (AG) 10 0 m g/kg和一氧化氮(NO)合成底物 L Arg10 0 mg/kg;观察给药前后血流动力学及组织氧分压变化 ,并记录存活时间和存活率。结果　与休克对照组〔分别为 (18.78± 4 .6 4 ) h和 10 %〕比较 ,L NAME组大鼠存活时间和 2 4 h存活率均无显著变化〔分别为 (2 3.80± 9.0 9) h和 4 0 % ,P均 >0 .0 5〕;AG组和 L Arg组大鼠存活时间均显著延长〔分别为 (2 8.72± 6 .2 5 ) h和 (30 .6 4± 8.77) h,P均 <0 .0 1〕,2 4 h存活率提高 (均为 80 % ,P均 <0 .0 1)。结论　选择性 i NOS抑制剂 AG及 L Arg对创伤性休克具有保护作用。Objective To investigate the influence of inhibitors of nitric oxide synthase (NOS) and L-arginine on the survival rate in a rat model of traumatic shock. Methods A rat model of traumatic shock was established by facturing the posterior limb of the rat. Nω-nitro-L-arginine-methyl ester (L-NAME, non-selective NOS inhibitor, 10 mg/kg), amino- guanidine〔AG, selective inducible nitric oxide synthase(iNOS) inhibitor, 100 mg/kg〕 and L-arginine 〔L-Arg, the precursor of nitric oxide(NO) synthesis 100 mg/kg〕 were injected intravenously during resuscitation,survival time and survival rate were observed. Results There were no significant changes in survival time and 24-hour survival rate between L-NAME 〔(23.80±9.09) hours and 40 %〕 and control group〔(18.78±4.64)hours and 10 %, both P>0.05〕; the survival time of AG group(28.72±6.25)hours and L-Arg group (30.64±8.77)hours prolonged apparently (both P<0.01), and 24-hour survival rate was also increased (both 80 %, both P<0.01). Conclusion Selective iNOS inhibitor AG and L-Arg exert benefitial effects on after traumatic shock rats.

关 键 词：休克 创伤性 一氧化氮 左旋精氨酸 氨基胍 

分 类 号：R965[医药卫生—药理学]