三倍体皱纹盘鲍(Haliotis discus Hannai)性腺发育的生物学研究  

THE BIOLOGY OF GONADIAL DEVELOPMENT OF TRIPLOIDY ABALONE (HALIOTIS DISCUS HANNAI)

作　　者：李霞[1] 阎松[1] 张国范[2] 王子臣[1] 

机构地区：[1]农业部海洋水产增养殖与生物技术重点开放实验室大连水产学院,大连116023 [2]中国科学院海洋研究所,青岛266071

出　　处：《海洋与湖沼》2004年第1期84-88,共5页Oceanologia Et Limnologia Sinica

基　　金：国家自然科学基金资助项目;39870 70 0号

摘　　要：采用光镜和电镜方法 ,观察了吊笼式人工养殖三倍体皱纹盘鲍性腺发育情况 ,并与二倍体进行比较。结果表明 ,三倍体鲍性腺发育分期基本与二倍体相同 ,但雄性腺发育停止在生长期 ,雌性腺少数可达成熟期 ,没有发现排放期。三倍体鲍性腺发育较二倍体差 ,表观看其性腺薄 ,只包围肝胰腺 1 / 3— 1 / 2 ,颜色浅。 7— 1 0月间检查的 86个二倍体个体中成熟期的占 2 4 4 % ,休止期占1 1 6 %。而 83个三倍体个体中 ,成熟期占 1 2 % ,休止期占 72 3%。三倍体成熟卵母细胞直径、核径、线粒体直径、胶膜厚度较二倍体大 ,但卵黄颗粒、线粒体。Contrary to most previous research,which focused on methods of inducing triploid,growth and hereditary features of abalone( Haliotis discus Hannai),this work investigated gonadal development of triploid and diploid disk abalone cultured artificially in cages with light microscopy and electromicrocopy. During 1997,triploid disk abalones were induced by caffeine and heat shock. The results showed that gonadal development stages of the triploid abalones were the same as that of the diploid,but testis development stopped during the growth period while the ovary developed to the mature stage. The spawning stage was not found. The triploid gonad was thin and light in colour,the length was only about the 1/3—1/2 length of the pancreance. The percentage of mature individuals were 24 4%and 11 6%in the resting period in diploid abalones. but 1 2% in the mature stage and 72 3% in the resting stage in triploid abalones. Gonad development of triploid was prevented. The diameter of the oocyte, nuclei, mitochondria of triploid abalone were longer than that of the diploid as was the jelly coat. The mount of yolk granula,however,the mitochondria and the endoplasmic reticulum of the oocyte in the triploidy gonad were less than that of the diploid. This may influence the development of early life and resuIt in larvae death. The structure of spermatic and spermiogenesis were observed in the diploid of abalones but no mature sperm in the testis of the triploid abalone was found. There was also a lot of deformed spermatid with abnormal cell organs,such as the mount of mitochondria and endoplasmic reticulum decreased and the lysosome increased and multiple holes in the nuclei. In addition,themitochondria was not arranged in a rose—like,style which,while distinct from Crassostrea gigas Thunberg and Pinctada martensii (Dunker),was common to that found in Saccostrea commercialis,Mya arenaria and Haliotis diversicolors.

关 键 词：三倍体皱纹盘鲍 性腺发育 生物学 人工养殖 

分 类 号：Q954.43[生物学—动物学]