Effects of temperature on UV-B-induced DNA damage and photorepair in Arabidopsis thaliana  

作　　者：LIShao-shan WANGYan BJOERNLarsOlof 

机构地区：[1]SchoolofLifeSciences,SouthChinaNormalUniversity,Guangzhou510631,China [2]SchoolofLifeSciencesandTechnology,JinanUniversity,Guangzhou510632,China [3]DepartmentofPlantPhysiology,LundUniversity,Box117,SE-22100Lund,Sweden

出　　处：《Journal of Environmental Sciences》2004年第1期173-176,共4页环境科学学报（英文版）

基　　金：TheNationalNaturalScienceFoundationofChina(No.30 2 0 0 0 30 ) ;theNaturalScienceFoundationofGuangdongProvinceandtheNaturalScienceFoundationofEducationalDepartmentofGuangdongGovernment

摘　　要：DNA damage in the form of cyclobutane pyrimidine dimers(CPDs) and (6-4) photoproducts(6-4PPs) induced by UV-B radiation in Arabidopsis thaliana at different temperatures was investigated using ELISA with specific monoclonal antibodies. CPDs and 6-4PPs increased during 3 h UV-B exposure, but further exposure led to decreases. Contrary to the commonly accepted view that DNA damage induced by UV-B radiation is temperature-independent because of its photochemical nature, we found UV-B-induction of CPDs and 6-4PPs in Arabidopsis to be slower at a low than at a high temperature. Photorepair of CPDs at 24℃ was much faster than that at 0℃ and 12℃, with 50% CPDs removal during 1 h exposure to white light. Photorepair of 6-4PPs at 12℃ was very slow as compared with that at 24℃, and almost no removal of 6-4PPs was detected after 4 h exposure to white light at 0℃. There was evidence to suggest that temperature-dependent DNA damage and photorepair could have important ecological implications.DNA damage in the form of cyclobutane pyrimidine dimers(CPDs) and (6-4) photoproducts(6-4PPs) induced by UV-B radiation in Arabidopsis thaliana at different temperatures was investigated using ELISA with specific monoclonal antibodies. CPDs and 6-4PPs increased during 3 h UV-B exposure, but further exposure led to decreases. Contrary to the commonly accepted view that DNA damage induced by UV-B radiation is temperature-independent because of its photochemical nature, we found UV-B-induction of CPDs and 6-4PPs in Arabidopsis to be slower at a low than at a high temperature. Photorepair of CPDs at 24℃ was much faster than that at 0℃ and 12℃, with 50% CPDs removal during 1 h exposure to white light. Photorepair of 6-4PPs at 12℃ was very slow as compared with that at 24℃, and almost no removal of 6-4PPs was detected after 4 h exposure to white light at 0℃. There was evidence to suggest that temperature-dependent DNA damage and photorepair could have important ecological implications.

关 键 词：Arabidopsis thaliana cyclobutane pyrimidine dimers(CPDs) DNA repair (6-4) photoproducts(6-4PPs) TEMPERATURE UV-B radiation 

分 类 号：Q754[生物学—分子生物学]