Effect of Zn_7-metallothionein on oxidative stress in liver of rats with severe thermal injury  

锌_7-金属硫蛋白对严重烫伤大鼠肝脏氧化应激反应的作用(英文)

作　　者：周智波[1] 丁海勤[1] 覃凤均 柳林 程时[1] 

机构地区：[1]北京大学医学部,生物物理系北京100083 [2]北京市积水潭医院烧伤科,北京100035

出　　处：《Acta Pharmacologica Sinica》2003年第8期764-770,共7页中国药理学报（英文版）

基　　金：Project supported by the Ministry of Health Foundation in China, № 96-1-278.

摘　　要：AIM: Using the model of burned animal with delayed resuscitation to study antagonistic effect of Zn_7-metallothionein (Zn_7-MT) on oxidative stress in the liver of rats suffered from severe thermal injury on skin. METHODS: To compare the changes in antioxidant concentrations and antioxidative enzyme activities in the liver or plasma of burned rats with or without Zn_7-MT in resuscitation fluid by biochemical assay. RESULTS: After injury, glutathione concentration was progressively decreased with time. At 24 h after injury, activities of glutathione reductase and glutathione peroxidase in the liver of burned rats were increased and then decreased at 48 h postburn. α-Tocopherol in plasma was reduced at 24 h and malondialdehyde in the liver was increased significantly postburn. MT and MT-1 mRNA expression in burned rats were activated. Taken together, oxidative stress in the liver of burned rats occurred. Exogenous Zn_7-MT attenuated the changes in antioxidant concentrations and antioxidative enzyme activities in the liver or plasma of burned rats. The effect of Zn_7-MT was in a concentration-dependent manner and the concentration of 10 μmol/L was the most effective. Exogenous Zn_7-MT also inhibited MT-1 mRNA overexpression and increased MT protein concentration. CONCLUSION: Zn_7-MT effectively antagonized oxidative stress in the liver of rats with severe thermal injury.目的:用大鼠皮肤严重烫伤及延迟复苏模型,观察锌_7-金属硫蛋白(Zn_7-MT)对烫伤后肝脏氧化应激的拮抗作用。方法:观察在复苏液中给予与不给予Zn_7-MT对烫伤后肝脏抗氧化物、抗氧化酶活性、脂质过氧化的变化以及内源性MT表达的影响。结果:伤后肝脏抗氧化物、抗氧化酶活性、脂质过氧化的变化以及内源性MT表达明显增强,说明肝脏有氧化应激反应。给予Zn_7-MT后,肝脏抗氧化物降低的幅度以及GSH-抗氧化酶活性下降的幅度减少,脂质过氧化部分被抑制,MT-1 mRNA高表达部分被抑制,Zn_7-MT的抗氧化应激作用呈剂量依赖性,浓度为10 μm01/L时效果最强。结论:外源性Zn_7-MT拈抗严重烫伤后肝脏氧化应激反应。

关 键 词：BURNS Zn_7-metallothionein LIVER oxidative stress 

分 类 号：R644[医药卫生—外科学]