急性百草枯中毒大鼠肺泡灌洗液及血清炎性因子变化及姜黄素的干预效果  

Influence of curcumin on changes of inflammatory factors in bronchoalveolar lavage fluid and serum in acute paraquat poisoned rats

作　　者：陈寿权[1] 冷巧云[2] 何爱文[1] 刘玉荣 李惠萍[1] 李章平[1] 黄唯佳[1] 程俊彦[1] 

机构地区：[1]温州医科大学附属第一医院急诊科,浙江温州325000 [2]九江市第一人民医院急诊科 [3]珠海市人民医院急诊科

出　　处：《灾害医学与救援（电子版）》2015年第4期207-211,共5页Disaster Medicine and Rescue(Electronic Edition)

基　　金：浙江省中医药科技计划项目(2010ZA082)

摘　　要：目的:观察百草枯(PQ)中毒大鼠急性肺损伤模型支气管肺泡灌洗液(BALF)和血清白介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)水平的变化及姜黄素的干预作用。方法:成年雄性SD大鼠随机分成对照组、中毒组、干预组。中毒组腹腔注射PQ 15 mg/kg,干预组腹腔注射姜黄素200 mg/kg,15 min后腹腔注射PQ 15 mg/kg,对照组腹腔注射生理盐水1 ml。各组分别按腹腔染毒或注射后3 h、6 h、24 h、3 d、7 d时间点分5个亚组。ELISA法测定各亚组大鼠BALF和血清IL-1β、TNF-α含量,光镜下观察各组大鼠肺组织病理变化。实验数据统计采用单因素方差分析,组间比较用Bonferroni法,相关性分析采用Pearson相关性检验。结果:与对照组比较,中毒组和干预组BALF和血清TNF-α、IL-1β水平在染毒后6 h、24 h、3 d、7 d均显著升高(P<0.05或P<0.01),而中毒组BALF的IL-1β水平在染毒后3 h已开始升高(P<0.01);与中毒组比较,干预组BALF和血清IL-1β和TNF-α水平在染毒后24 h、3 d、7 d均显著下降(P<0.05或P<0.01);中毒组、干预组BALF与血清IL-1β、TNF-α水平具有相关性。中毒组病理改变严重,干预组病理改变较中毒组减轻。结论:PQ中毒大鼠肺组织炎性细胞浸润,BALF和血循环中TNF-α、IL-1β等炎性因子释放增加,启动肺及全身炎症反应,可能是大鼠PQ中毒急性肺损伤的基本机制之一,姜黄素可通过此信号通路在一定程度上减轻肺损伤和全身炎性反应。Objective:To study the changes of interleukin-1β(IL-1β) and tumor necrosis factor-α(TNF-α) in bronchoalveolar lavage fluid(BALF) and serum in rats with acute paraquat(PQ) poisoning,and the intervention effect of curcumin.Methods:A total of 120 adult male Sprague-Dauley(SD) rats were randomly divided into control,PQ-poisoned and curcumin-intervened groups.Rats in PQ-poisoned group,curcumin-intervened group,control group were intraperitoneally administered with PQ 15 mg/kg,PQ 15 mg/kg at 15 min after curcumin 200 mg/kg,normal saline(NS) 1 ml,respectively.Each group divided further into 5 subgroups by time points at 3h,6h,24 h,3d,7d after intraperitoneal contamination or injection.The levels of IL-1β,TNF-α in serum and BALF were measured with enzyme-linked immunosorbent assay(ELISA).Pathological changes in lung were observed by light microscope.Experimental data were treated with single-factor analysis of variance and compared between groups by Bonferroni method,and correlation analysis was performed by Pearson correlation coefficient.Results:Compared with the control group,TNF-α and IL-1β levels in BALF and serum increased significantly at 6 h,24 h,3 d,7 d after PQ exposure in PQ-poisoned group and curcumin-intervened group(P<0.05 or P<0.01),and IL-1β levels in BALF of PQ-poisoned group had increased significantly at 3h after PQ exposure(P<0.01).Compared with the PQ-poisoned group,TNF-α and IL-1β levels in BALF and serum decreased significantly at 24 h,3 d,7 d after PQ exposure in curcumin-intervened group(P<0.05 or P<0.01).The levels of IL-1β and TNF-α were relevant between BALF and serum in PQ-poisoned group and curcumin-intervened group.Pathological changes of lung tissue were more serious in PQ-poisoned group than curcumin-intervened group.Conclusion:Neutrophil infiltration in lung tissue,increased releasing of IL-1βand TNF-α in BALF and blood circulation,and then initiating pulmonary and systemic inflammatory responses could be one of the basic mechanisms of PQ-induced acute lung injury in rats.

关 键 词：百草枯 中毒 肺损伤 炎性因子 姜黄素 

分 类 号：R595.4[医药卫生—内科学]