生物检材中乌头碱的GC/MS分析  

Detection of aconitine in biological samples by GC/MS

作　　者：王朝虹[1] 何毅[1] 张继宗[1] 胡春华[1] 果德安[2] 

机构地区：[1]公安部物证鉴定中心,北京100038 [2]北京大学药学院,北京100083

出　　处：《中国法医学杂志》2003年第3期145-146,153,共3页Chinese Journal of Forensic Medicine

基　　金：公安部重点课题基金(编号 20018223301)

摘　　要：目的 建立GC/MS-SIM检测生物检材中乌头碱的定性方法。方法 以MSTFA作为衍生化试剂,吡啶为溶剂,于60℃反应30min,综合质谱图及保留时间定性分析。结果 经该方法测得乌头碱最小检出量为20ng(S/N≥100)。结论 GC/MS-SIM法适用于药材及生物检材中乌头碱的定性分析。Objective To establish a method used for qualitative determination of aconitine in biological samples by GC/MS. Methods using MSTFA as derivative reagent and pyridine as solvent leaving in 60℃ for 30min then detected by GC/MS-SIM. Results The limit of detection of aconitine was 20ng (S/N≥100). Conclusion This method is sensitive, fast, and accurate. It is useful for forensic medical practice.

关 键 词：生物检材 乌头碱 GC/MS 法医毒物分析 

分 类 号：D919[医药卫生—法医学]