辣椒轻斑驳病毒葫芦岛分离物RNA杂交检测体系的建立  

Establishment of RNA hybridization detection system for pepper mild mottle virus

作　　者：周涛 于曼 张鑫宇 安梦楠[1] 吴元华[1] Zhou Tao;Yu Man;Zhang Xinyu;An Mengnan;Wu Yuanhua(College of Plant Protection, Shenyang Agricultural University, Shenyang Liaoning 110866, China)

机构地区：[1]沈阳农业大学植物保护学院,辽宁沈阳110866

出　　处：《中国植保导刊》2018年第12期8-13,共6页China Plant Protection

基　　金：农业部/浙江省植保生物技术重点实验室开放课题;国家自然科学基金(31401710)

摘　　要：辣椒轻斑驳病毒(pepper mild mottle virus,PMMoV)可引起辣椒叶片以及果实的花叶和畸形症状,造成相当的经济损失。为了建立辣椒轻斑驳病毒的高特异性和灵敏度的分子检测体系,采用非放射性的化合物地高辛(DIG)标记检测PMMoV正义链的RNA探针,建立了该病毒Dot blot杂交和Northern blot杂交检测体系,并通过RT-PCR法验证了杂交体系的检测特异性。结果表明,RNA探针对PMMoV具有很高的检测特异性和灵敏度,适用于病毒的早期检测以及相关分子研究。Pepper mild mottle virus(PMMoV) causes mosaic symptoms and malformation on both leaf and fruit of pepper and considerably reduces economical yields. Dot blot and Northern blot hybridization detection systems for PMMoV Huludao isolates were established using non-isotopic digoxigenin labeled positive-strand RNA probes. The detection specificity was verified by RT-PCR methods. In this study, PMMoV RNA detection probes showed high detection specificity and sensitivity, which was applicable in early detection and molecular study on PMMoV.

关 键 词：辣椒轻斑驳病毒 特异性检测 DOT blot杂交 NORTHERN blot杂交 RNA探针 

分 类 号：S436.418.12[农业科学—农业昆虫与害虫防治]