番茄叶霉病菌对多菌灵、乙霉威及代森锰锌抗性检测  

Detecting the Resistance of Fulvia fulva to Carbendazim, Diethofencarb and Mancozeb

作　　者：王美琴[1] 刘慧平[1] 韩巨才[1] 高俊明[1] 

机构地区：[1]山西农业大学农学院,山西太谷030801

出　　处：《农药学学报》2003年第4期30-36,共7页Chinese Journal of Pesticide Science

基　　金：山西省留学基金资助项目(99051);山西省科技攻关项目(031011-4);山西省科技攻关项目(033006).

摘　　要：报道了番茄叶霉病菌Fulviafulva对多菌灵、乙霉威及代森锰锌的敏感性基线,以及抗性频率和抗性水平。离体条件下,多菌灵、乙霉威及代森锰锌对番茄叶霉病菌敏感菌株的平均EC50值分别为0.101、2.475、9.067μg/mL;最低抑制浓度(MIC)值分别为0.5、5、50μg/mL。山西晋南地区番茄叶霉病菌对3种杀菌剂的抗性频率最高,分别达到97.4%、70.5%、98.7%;山西吕梁地区与太原地区相对较低,但该病菌对3种杀菌剂的抗性频率也都超过了30%。辽宁沈阳、山东寿光、河北保定番茄叶霉病菌对多菌灵的抗性频率均为100%;对乙霉威的抗性频率前两地为100%,保定为10%;对代森锰锌的抗性频率都超过90%。所有抗性菌株对多菌灵均属于高抗类型,抗性指数超过5000,测不出MIC值;对乙霉威有50%的高抗菌株,抗性指数在100以上;对代森锰锌各地均未发现高抗菌株,低抗和中抗菌株所占比例较大,其抗性指数集中在50左右。对多菌灵与乙霉威具有双重抗性的菌株占测试菌株总数的49.9%,并且首次在田间发现了对3种杀菌剂都具有抗性的番茄叶霉病菌多抗菌株。The sensitive baseline of Fulvia fulva (Cooke)Ciferri to carbendazim, diethofencarb and (mancozeb,)the resistant frequencies and the resistant level were presented. The minimum inhibitory (concentration)(MIC)of isolates to the three fungicides were 0.5,5 and 50 μg/mL,respectively; EC(50) were (0.101,)2.475 and 9.067 μg/mL,respectively. These data could be taken as relative sensitive baseline because no fungicide had been used before in that area.. The resistant frequencies of isolates from Shanxi Lüliang and Taiyuan (regions) were relatively lower,but both of them were more than 30%. North and south parts of Shanxi Province were higher than 70%. The resistant frequencies of Shenyang,Shouguang Shandong and Baoding Hebei to the three fungicides were high, except the isolates to diethofencarb (10%) in Baoding Hebei. The resistant levels of all detected isolates were the highest to carbendazim,more than 5 000 times. It was impossible to measure MIC value;The resistant levels of 50% isolates were high resistance,and they were more than 100 times. Most of isolates to mancozeb belonged to low level resistance and middle level (resistance,) and the (resistant) levels were about 50 times. 49.9% isolates have developed resistance to (carbendazim) and (diethofencarb.) Besides,multiple resistant. Isolates (resistance to (carbendazim)、diethofencarb and mancozeb )were found in the filed.

关 键 词：番茄叶霉病菌 多菌灵 乙霉威 代森锰锌 抗性检测 

分 类 号：S436.412[农业科学—农业昆虫与害虫防治] S481.4[农业科学—植物保护]