不同品系小型猪对高致病性猪繁殖与呼吸综合征病毒的敏感性筛选  

Susceptibility screening of highly pathogenic porcine reproductive and respiratory syndrome virus in several strains of minipigs

作　　者：潘金春[1,2] 任柏桦 闵凡贵[1,2] 陈瑞爱[3,4] 王希龙[1,2] 王林川[3,4] 王凤国 罗书明[3] 叶健聪[3] 刘玲[3] 黄鹏华 

机构地区：[1]广东省实验动物监测所,广东广州510663 [2]广东省实验动物重点实验室,广东广州510663 [3]广东大华农动物保健品股份有限公司,广东新兴527400 [4]华南农业大学兽医学院,广东广州510642

出　　处：《中国比较医学杂志》2015年第6期14-17,共4页Chinese Journal of Comparative Medicine

基　　金：广东省科技计划项目(2012B060300001);广东省教育部产学研结合项目(2012B091100466)

摘　　要：目的筛选对高致病性猪繁殖与呼吸综合征病毒(HP-PRRSV)敏感的小型猪品系,以用于HPPRRS活疫苗的评价。方法本研究选择蓝塘猪、蕨麻小型猪、巴马小型猪、五指山小型猪(白系)接种PRRSV NVDC-JXA1株强毒,并以本地二元杂猪作为对照。攻毒后每天观察动物精神、食欲、死亡等情况,死亡动物进行剖检,并采取肺组织做RT-PCR检测病毒,连续观察5周,试验结束时采取存活动物血清分别检测PRRSV抗体。结果攻毒后各组都有动物出现精神沉郁、食欲下降、死亡等现象,死亡动物RT-PCR检测都显示为阳性,存活动物PRRSV抗体检测阳性。从死亡率看,巴马小型猪和五指山小型猪白系对PRRSV NVDC-JXA1株强毒的敏感性最高,并且敏感性超过了对照组二元杂猪。结论巴马小型猪和五指山小型猪白系对HP-PRRSV敏感,可用于HPPRRS活疫苗的检验。Objective To screen strains of minipigs sensitive to highly pathogenic porcine reproductive and respiratory syndrome virus( HP-PRRSV) for evaluation of HP-PRRS live vaccine. Methods Lantang pigs,Juema,Bama and Wuzhishan( white) minipigs were inoculated with virulent strain NVDC-JXA1 of PRRSV,and local binary hybrid pigs were used as control. The animals were continuously observed for 5 weeks on mental status,appetite,survival,etc. after inoculation of virus. The dead pigs were autopsied and the lung tissue samples were collected for detecting virus by RTPCR. By the end of the experiment,serum of survival animals were collected for detecting PRRSV antibody by ELISA assay. Result The animals showed depression, anorexia, and other clinical signs and death in each group after inoculation. Meanwhile,the testing results were all positive in the RT-PCR and ELISA detection. Bama and Wuzhishan( white) minipigs were the most sensitive to virulent strain NVDC-JXA1 of PRRSV regarding mortality rate. Conclusions Bama and Wuzhishan( white) minipigs are sensitive to HP-PRRSV,and can be used for the inspection of HP-PRRS live vaccine.

关 键 词：小型猪 高致病性猪繁殖与呼吸综合征病毒 敏感性筛选 

分 类 号：S852.65[农业科学—基础兽医学]