黄芪多糖对雏鸡黏膜sIgA含量的影响研究  

Effect of Astragalus Polysaccharides on Mucosal s Ig A Content in Chicks

作　　者：刘瑞[1] 于群[1,2] 李莹[1] 赵霞[1] 张瑞莉[1] 葛铭 

机构地区：[1]东北农业大学动物医学学院,黑龙江哈尔滨150030 [2]中国农业科学院哈尔滨兽医研究所,黑龙江哈尔滨150001

出　　处：《中国家禽》2014年第21期23-26,共4页China Poultry

基　　金：黑龙江省自然科学基金项目(C201222);国家自然科学基金项目(31272533)

摘　　要：为研究黄芪多糖(APS)对雏鸡黏膜分泌型Ig A(s Ig A)分泌的影响,将240只7日龄雏鸡随机分为4组:Ⅰ、Ⅱ、Ⅲ和C组,其中,Ⅰ、Ⅱ、Ⅲ组雏鸡于7日龄以灌服方式分别给予80 mg/m L、40 mg/m L、20 mg/m L APS液各0.2 m L,1次/d,连续5 d,C组雏鸡以相同方式给予相同剂量的生理盐水,于14日龄采取滴口方式进行鸡传染性法氏囊病活疫苗免疫,在首次给药后1、3、5、7 d以及疫苗免疫后7、14、21、28、35、42、49 d,每组随机抽取5只雏鸡,收集泪液后,心脏采血处死,快速收集气管液、肠液,采用间接ELISA检测s Ig A含量。结果显示,Ⅰ、Ⅱ、Ⅲ组雏鸡s Ig A含量与C组差异显著(P<0.05或P<0.01),泪液中s Ig A含量Ⅰ组雏鸡相对较高,气管液、肠液中s Ig A含量Ⅲ组雏鸡相对较高,提示APS对雏鸡黏膜s Ig A分泌有显著的促进作用。In order to study the effects of astragalus polysaccharides(APS)on secretory immunglobulin A(s Ig A)secretion in chicken mucosa,240 of 7-day-age chickens were randomly divided into four groups in the experiment.Group Ⅰ,Ⅱ,Ⅲ were respectively given APS one time daily with a volume of 0.2 m L at a dosage of 80,40 and20 mg/m L(P.O.)for 5 days,and group C were given physiological saline in the same way. At the 14-day-age the chickens were vaccinated with infectious bursal disease moderate virulence live vaccine. On the 1st,3rd,5th and 7th day after the first treatment and 7th,14 th,21st,28 th,35th,42 nd and 49 th day after inoculation,5 chickens were selected randomly from each group and collected tears,then executed by drawing-out blood in hearts,collected tracheal liquid and intestinal juice immediately,and the s Ig A contents of the liquids were detected by ELISA. The results indicatedthat the s Ig A contents of group Ⅰ,Ⅱ and Ⅲ were significantly different from control group(P<0.05 or P<0.01). In tears,the indexes of group Ⅰ were relatively higher than other groups,while in tracheal liquid and intestinal juice the indexes of group Ⅲ were relatively higher than the other two groups. Therefore,it suggested that APS could significantly elevate s Ig A secretion of mucosa.

关 键 词：黄芪多糖 雏鸡 黏膜免疫 SIGA 

分 类 号：S852.4[农业科学—基础兽医学]