紫色叶用莴苣遗传多样性及亲缘关系的TRAP分析  

Analysis of Genetic Diversity and Relationship in Purple Lettuce by TRAP Technique

作　　者：王亚楠[1] 韩莹琰[1] 范双喜[1] 卜斌[1] 

机构地区：[1]北京农学院植物科学技术学院,北京102206

出　　处：《中国蔬菜》2015年第3期25-32,共8页China Vegetables

基　　金：国家自然科学基金项目(31372057);现代农业产业技术体系北京市叶类蔬菜创新团队建设专项(blvt-02);北京市科技新星计划项目(2010B020)

摘　　要：利用TRAP标记对47份紫色叶用莴苣种质进行亲缘关系及遗传多样性分析。20对引物组合共扩增出多态性条带430条,多态性比率(PPB)为67.08%。47份紫色叶用莴苣材料的遗传相似系数在0.7063~0.9828之间,Nei’s基因多样性指数(He)和Shannon’s信息指数(I)分别为0.2553和0.3976,遗传多样性水平较低。聚类分析结果表明,47份紫色叶用莴苣材料分为2大类群,第Ⅱ类群又分为5个亚类,叶片形态相似的种质基本上聚在一起,亲缘关系较近。TRAP technique was used to analyze the genetic diversity and relationship of 47 purple lettuce (Lactuca sativa L. )species.20 pairs of TRAP primers amplified polymorphic bands were 430,and the polymorphic rate was 67.08%.The genetic similarity coefficient was 0.706 3-0.982 8.The average Nei’s gene diversity index and Shannon’s information index were 0.255 3 and 0.397 6,respectively,indicating that the low degree of genetic diversity in purple lettuce.The cluster analysis divided these 47 species into 2 major groups, and the Ⅱgroup could be devided into 5 subgroups.The species with similar leaf morphology were clustered together,they had closer genetic relationship.

关 键 词：紫色叶用莴苣 TRAP标记 遗传多样性 

分 类 号：S636.2[农业科学—蔬菜学]