野生小鼠来源1号染色体替换系小鼠的生长表型与血液生化检测  

Detection of growth phenotype and blood biochemical parameters of wild type-derived chromosome 1 substitution mouse strain

作　　者：高遄 徐伟 徐福意 张耀奇 赵莹 赵丽亚 周宇荀[1] 李凯[1] 肖君华[1] 

机构地区：[1]东华大学化学化工与生物工程学院,上海201620 [2]上海实验动物研究中心,上海201203

出　　处：《中国实验动物学报》2015年第3期233-238,共6页Acta Laboratorium Animalis Scientia Sinica

基　　金：国家自然科学基金(编号:31171199);上海市创新行动实验动物研究项目(编号:11140900200;13140900300);中央高校基本科研业务费专项资金;东华大学"励志计划"(B201308)

摘　　要：目的分析野生小鼠来源1号染色体替换系小鼠群体的生长表型与血液生化指标,探讨替换系群体的QTL定位潜力。方法以染色体替换系近交后代小鼠为研究对象,测定其体重、体长、尾长、血液生化、内脏器官重量等表型数据,统计其与C57BL/6差异显著性。结果发现替换系小鼠在多个指标上与C57BL/6具差异有显著性。不同发育时期的外部表型上与C57BL/6相比,体重具显著差异的是B6-Chr1KM等6个品系,体长具显著差异的是B6-Chr1K M等5个品系,尾长具显著差异的是B6-Chr1K M等9个品系;部分品系在肝、脾、肾脏和脑部重量上存在与C57BL/6的显著差异;生化指标上,丙氨酸氨基转移酶B6-Chr1CM雌鼠显著偏高,碱性磷酸酶B6-Chr1HZ雌鼠显著偏高、B6-Chr1KM雄鼠显著偏低,总胆红素B6-Chr1CM雄鼠、B6-Chr1SMX雄鼠和B6-Chr1HZ雄鼠显著偏高,甘油三酯B6-Chr1SMX雄鼠显著偏高,总胆固醇B6-Chr1TW雄鼠显著偏高。结论培育的野生小鼠来源的1号染色体替换系群体在部分表型上与C57BL/6差异有显著性,具备作为QTL定位的遗传资源潜力。Objective To analyze the growth phenotype and blood biochemical parameters of chromosome 1 substitution mouse strain( CSS1),and investigate their potential of QTL mapping. Methods Body weight,body length,tail length,organ weight of the CCS1 mice were measured at different days to create a growth curve while blood biochemical indexes were measured at about the 80 th day. Results The CCS1 mice were different from C57 BL /6 mice in several indexes. Compared with the C57 BL /6 mice during different developmental stages,six strains including B6-Chr1 KMmice were significantly different in body weight. There were five strains including B6-Chr1 CMmice significantly different with C57 BL /6 mice in body length,and all of the CSS1 mice were significantly different from C57 BL /6 mice in tail length. Part of CCS1 mice were significantly different from C57 BL /6 mice in the weight of liver,spleen,kidney and brain. The ALT of female B6-Chr1 CMmice was significantly higher than that in the C57 BL /6 mice. The ALP of female B6-Chr1 HZmice was significantly higher than that in the male C57 BL /6 and B6-Chr1 KMmice,and was significantly lower than that in the C57 BL /6mice. The TB of male B6-Chr1 CM,B6-Chr1 SMXand B6-Chr1 HZmice was significantly higher than that of the C57 BL /6mice. The TG of male B6-Chr1 SMXmice and male B6-Chr1 TWmice was significantly higher than that in the C57 BL /6 mice.Conclusions The phenotype of Chr1 CSS mice is quite different from commonly used inbred strain C57 BL /6 mice. CCS1 mice show great potential in QTL mapping for their characteristic growth phenotype and blood biochemical indexes.

关 键 词：近交系小鼠 野生小鼠 生长发育 血液生化 脏器系数 染色体替换系小鼠 

分 类 号：R285.5[医药卫生—中药学]