传统中药补肾固筋方对膝骨性关节炎IL-1、TNF-α的作用机制  

Effect of the traditional Chinese medicine Bushen Gujin prescription on the expression of interleukin-1 and tumor necrosis factor-α in rabbit models of knee osteoarthritis

作　　者：张冲[1,2] 王恒树[1,2] 阚秀莲 李健 郭梅 祁永玲 韩艳丽 杨瑞成[1,2] 

机构地区：[1]河北省中医院骨三科 [2]河北医科大学第二医院,石家庄050011

出　　处：《中国实验动物学报》2015年第3期312-315,共4页Acta Laboratorium Animalis Scientia Sinica

基　　金：河北省中医药类科研计划指导性课题(No.2014172)

摘　　要：目的观察补肾固筋方对兔膝骨关节炎(knee osteoarthritis,KOA)血清及关节液IL-1、TNF-α的表达影响及调节机制。方法新西兰兔76只,随机分为正常组、模型组、西药组、中药组,除正常组外均用改良Hulth法造,取血清及膝关节前方部分滑膜组织、股骨内髁关节软骨及软骨下骨,ELISA法IL-1、TNF-α含量。结果西药组关节间隙狭窄,介于模型组和中药组之间,关节面粗糙,骨赘明显;中药组关节间隙稍窄,关节面稍粗糙伴轻微骨赘。模型组血清及关节液IL-1、TNF-α含量比正常组升高(P<0.01)。两个给药组血清及关节液IL-1、TNF-α含量比模型组降低(P<0.01),两给药组之间差异无显著性。结论补肾固筋方通过降低骨关节炎中IL-1、TNF-α的分泌水平促进软骨修复,为临床应用提供科学的实验依据。Objective To explore the effect of traditional Chinese medicine( TCM) Bushen Gujin prescription on the expression of interleukin-1( IL-1) and tumor necrosis factor-α( TNF-α) in the serum and synovial fluid of rabbit models of knee osteoarthritis. Methods Seventy-six healthy 8-month old male SPF New Zealand white rabbits were randomly divided into normal,model,western medicine( meloxicam) and TCM groups( n = 19 in each group). The rabbit models of knee osteoarthritis were generated by Hurth procedure,and were given respective drug treatment for consecutive 8 weeks.The expression of IL-1 and TNF-α in serum and synovial fluid was detected by ELISA assay. Results All the experimental rabbits showed narrowed knee articular space,rough articular cartridge surface,and obvious bone spur formation. The width of articular space of the meloxicam-treated rabbit models was between those of the model group and Chinese medicinetreated group. The expression of IL-1 and TNF-α in the serum and synovial fluid was increased in the rabbits of model groups,decreased in the two drug-treated groups( both P < 0. 01),but not significantly different between the two drugtreated groups( P > 0. 01). Conclusions The traditional Chinese medicine Bushen Gujin prescription can effectively inhibit synovial inflammation and reduce the expression of IL-1 and TNF- in the serum and synovial fluid. Our results provide experimental support for the use of this Chinese medicine in the treatment of knee osteoarthritis in clinics.

关 键 词：兔膝骨性关节炎 补肾固筋方 关节滑液 白细胞介素-1 肿瘤坏死因子-Α 治疗 

分 类 号：R285.5[医药卫生—中药学]