珍稀泌盐植物长叶红砂(Reaumuria trigyna)愈伤组织诱导及增殖  

Callus Induction and Proliferation in the Endemic Recretohalophyte Reaumuria trigyna

作　　者：党振华[1] 秦晓春[2] 王迎春[1] 

机构地区：[1]内蒙古大学生命科学学院牧草与特色作物生物技术重点实验室,内蒙古呼和浩特010021 [2]包头市园林科技研究所,内蒙古包头014010

出　　处：《中国沙漠》2015年第5期1262-1267,共6页Journal of Desert Research

基　　金：国家自然科学基金项目(31360063);内蒙古自治区高等学校创新研究团队发展计划项目(NMGIRT1401)

摘　　要：以珍稀泌盐植物长叶红砂(Reaumuria trigyna)成熟种子和不同发育阶段幼苗的不同部位为外植体,对其愈伤组织诱导和增殖进行了系统研究。结果表明:在添加3.0mg·L-12,4-D、1.0mg·L-1 NAA、0.5mg·L-1 6-BA、30g·L-1蔗糖和6.0g·L-1琼脂,pH为5.8的MSO培养基中,出愈率达70%以上,生长量高于其他培养基,是该植物愈伤组织诱导的最佳培养体系;7d下胚轴和25d的幼苗较适合愈伤组织诱导,可形成淡黄色疏松愈伤组织,且褐化程度相对较低;适宜的继代周期为30~40d,随着传代次数增加,愈伤组织颜色变淡,生长速度加快;NaCl浓度为50mmol·L-1和100mmol·L-1时促进愈伤组织生长,盐浓度过高则导致其生长受抑,甚至死亡。The aim of this study was to evaluate callus induction and proliferation in the endangered recretohalophyte Reaumuria trigyna.Mature seeds and seedlings at different development stages were used as the ex-plant materials.The best medium for callus formation and proliferation was improved MSO(pH5.8)supplemented with 3.0 mg·L-1 2,4-dichlorophenoxyacetic acid,1.0 mg·L-1 naphthaleneacetic acid,0.5 mg·L-1 6-benzylaminopurine,30 g·L-1 sucrose,0.5 g·L-1 casein hydrolysate,and 6.0 g·L-1 agar.On this medium,the callus induction rate was greater than 70%,and the growth rate was higher than those of ex-plants on other media.The optimal ex-plants for callus induction were hypocotyls from7-day-old and 25-day-old seedlings.The calli that formed from these ex-plants were loose,light yellow,and showed a lower browning rate,compared with calli that grew from other ex-plants.The optimum duration of the subculture cycle was 30-40 days.With longer passage times,the callus grew faster and was a lighter color.When calli were cultured on media with different NaCl concentrations,their growth was accelerated on media containing 50 and 100 mmol·L-1 NaCl,but higher concentrations of NaCl inhibited callus growth or resulted in callus death.

关 键 词：长叶红砂(Reaumuria trigyna) 愈伤组织 诱导 增殖 

分 类 号：Q943.1[生物学—植物学]