一株兔病毒性出血症病毒野毒WF株的分离与鉴定  

Isolation and Identification of a Strain of Wild-type WF RHDV

作　　者：李天芝 于新友[1] 王艳[2] 沈志强[2] 

机构地区：[1]山东省绿都生物科技有限公司,滨州256600 [2]山东省滨州畜牧兽医研究院,滨州256600

出　　处：《中国养兔》2015年第4期8-10,共3页Chinese Journal of Rabbit Farming

基　　金：山东省现代产业技术体系毛皮动物创新团队项目(SDAIT-18-011-15)

摘　　要：对山东省潍坊某兔场送检的疑似兔病毒性出血症病料进行细菌学检验、血凝性、特异性鉴定,并进一步进行毒力鉴定和免疫原性测定。结果显示:RHDV WF株对人"O"型红细胞具有高度血凝性,血凝效价为1∶2048,RHDV抗血清可特异性抑制RHDV WF株对人"O"型红细胞的凝集,RHDV WF株对家兔的LD50为10-6.5/m L,是一株对家兔具有高致病力的强毒株。取第5代RHDV WF株制备灭活苗,用1.0mL免疫试验兔,免疫后第14天攻毒,对RHDV的保护率达100%。A strain of rabbit hemorrhagic disease virus was isolated in a farm,and was named WF strain.A se?ries of tests such as bacteriological examination HA,specificity,virulence and immunogenicity were detected. The results showed that the strain of WF RHDV had high HA activity to human group O red blood cells the va?lence could reach to 1:2048.The serum against RHDV could restrain this activity the LD50 of RHDV to rabbits was 10-6.5/mL,the WF RHDV was a virulent strain to rabbits. The inactivated vaccine of 1 mL made by the fifth generation of RHD WF strain was used to immunize rabbits. The immune protection rate against RHD was 100%on the fourteenth days after vaccination.

关 键 词：兔出血症病毒 分离 鉴定 

分 类 号：S858.291[农业科学—临床兽医学]