橄榄苦苷对成骨细胞OPG/RANKL mRNA的影响  

Effects of oleuropein on OPG/RANKL mRNA expression in osteoblasts

作　　者：刘慧兰[1] 黄惠娟[1] 李宝恒[1] 吴冬[1] 

机构地区：[1]厦门大学附属东方医院,福州350025

出　　处：《中国骨质疏松杂志》2015年第6期649-652,共4页Chinese Journal of Osteoporosis

基　　金：军区医学科技创新课题(11M111)

摘　　要：目的通过测定橄榄苦苷对成骨细胞OPG/RANKL mRNA的表达,探讨其治疗骨质疏松症的机制。方法运用荧光定量PCR技术(RT-PCR)检测不同浓度橄榄苦苷不同时间对成骨细胞OPG/RANKL mRNA表达量。结果与阴性对照组比较,RT-PCR结果显示不同浓度的橄榄苦苷均能促进OPG mRNA的表达,并且抑制成骨细胞RANKL mRNA的表达。结论橄榄苦苷可能通过抑制RANKL分泌来降低破骨细胞活性,同时促进成骨细胞分泌OPG,使之与RANKL结合增多,间接作用于破骨细胞,使其活性降低,而达到治疗骨质疏松的目的。Objective To explore the mechanism of oleuropein in the treatment of osteoporosis by measuring the expression of OPG / RANKL m RNA in osteoblasts. Methods The expression of OPG / RANKL m RNA in osteoblasts was detected using fluorescence quantitative PCR( RT-PCR) in the presence of different solubility of oleuropein at different time. Results Compared to the control group,the results of RT-PCR showed that oleuropein of all concentrations promoted the expression of OPG m RNA,and inhibited the expression of RANKL m RNA. Conclusion Oleuropein reduces the activity of osteoclasts by inhibiting the secretion of RANKL,and simultaneously promotes osteoblasts to secrete OPG,which combines with RANKL that indirectly affects osteoclasts,leading to the decrease of osteoclast activity and reaching the purpose of the treatment of osteoporosis.

关 键 词：橄榄苦苷 成骨细胞 OPG/RANKL MRNA 影响 

分 类 号：R580[医药卫生—内分泌]