白藜芦醇及与环孢菌素A联用对人外周血T细胞免疫功能的影响  

Effect of resveratrol alone and its com- bination with cyclosporin A on the im- mune function of human peripheral blood T lymphocytes

作　　者：于良[1] 吴胜利[1] 张梅[1] 潘承恩[1] 

机构地区：[1]西安交通大学第一医院肝胆外科,陕西西安710061

出　　处：《细胞与分子免疫学杂志》2003年第6期549-551,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：陕西省自然科学基金资助项目(No.2002C249)

摘　　要：目的:研究不同浓度白藜芦醇(Res)及与环孢霉素A(CsA)联用时,对人外周血T细胞(hPBTC)的增殖、淋巴细胞母细胞化及IL-2、INF-γ产生的影响。方法:采用MTT比色法检测hPBTC的增殖。形态学方法观测T细胞转化率。ELISA法检测淋巴细胞培养上清液中IL-2、INF-γ含量。结果::Res的浓度>2.5 mg/L时,hPBTC增殖及淋巴细胞的转化率均明显降 低(P<0.05),以10 mg/L的效果最为显著;Res浓度>5 mg/L时,培养上清液中IL-2和INF-γ的含量下降明显(P<0.05)。Res浓度>2.5 mg/L时与CsA联用具有协同作用。结论:Res对人淋巴细胞的增殖及母细胞化具有明显的抑制作用,在一定的浓度范围内其与CsA联合应用能增强免疫抑制作用。AIM: To investigate the effect of resveratrol (Res) alone and its combination with cyclosporin A (CsA) on the proliferation of human peripheral blood T lymphocytes (hPBTCs), transformation into lymphoblasts, as well as IL-2 and INF-y production. METHODS: The proliferation of hPBTCs was detected by MTT colorimetry. The lymphocyte transformation rate was examined by morphologic method. The levels of IL-2 and INF-y in lymphocyte culture supernatant were detected by ELISA. RESULTS: When the concentration of resveratrol was over 2. 5 mg/L, especially 10 mg/L, the proliferation and transformation rate of hPBTCs decreased significantly ( P < 0. 05). The levels of IL-2 and INF-y in lymphocyte culture supernatant reduced markedly (P<0.05), on condition that the concentration of resveratrol was over 5 mg/L. The combination of more than 2. 5 mg/L of resveratrol with cyclosporine A had synergism. CONCLUSION: Resveratrol can suppress notably the proliferation and transformation of human lymphocytes and the combination of resveratrol at a given concentration with cyclosporine A can enhance immune suppression.

关 键 词：白藜芦醇 环孢霉素A 免疫抑制 T细胞 细胞因子 

分 类 号：R622.3[医药卫生—整形外科]