人同种异体抗原诱导的T细胞克隆在体外的建立及鉴定  

Establishment and characterization of human alloantigen-induced T cell clones

作　　者：韩卫宁[1] 曹云新[1] 张赟[1] 李琦[1] 谢鑫[1] 刘雪松[1] 金伯泉[1] 

机构地区：[1]第四军医大学基础部免疫学教研室,陕西西安710032

出　　处：《细胞与分子免疫学杂志》2003年第6期541-543,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金资助项目(No.3000150)

摘　　要：目的:在体外建立长期培养的人T细胞克隆。方法:以60Co照射的无关PBMC作为饲养细胞,在条件培养液中以有限稀释法对经单向混合淋巴细胞反应活化的T细胞进行克隆化。用免疫荧光染色和流式细胞术分析鉴定所获克隆。结果:获得的16株克隆中,除1株外,均为αβT细胞。15株αβT细胞克隆中,有9株为Th(3株Th0、6株Th2),6株为Tc(5株Tc0,1株Tc2)。结论:建立了15株稳定的T细胞克隆并进行了鉴定,为Tc1和Tc2亚群标记和功能的研究打下了基础。AIM: To estabilish and analyze stable human T cell clones cultured in vitro. METHODS: The activated T cells from mixed lymphocyte culture (MLC) were cloned by limiting dilution using irradiated PBMCs as feeder cells. T cell clones were then characterized by immunofluorescence staining and flow cytometry analysis. RESULTS: 16 clones were obtained, of which all but one were αβpT cells. Among the 15 αβT cell clones, 9 clones belonged to Th subset, including 3 ThO and 6 Th2, and 6 clones belonged to Tc subset, including 5 Tc0 and 1 Tc2. CONCLUSION: Fifteen human T cell clones were successfully established, which lays the foundation for study on markers and function of Tc1 and Tc2 subsets.

关 键 词：克隆 Tc1 Tc2 

分 类 号：R392.11[医药卫生—免疫学]