表面增强拉曼光谱法快速分析中药三七的有效成分  

Rapid analysis of active ingredients in Panax notoginseng using surface enhanced Raman spectroscopy

作　　者：卫程华 陈娟[1] 许曼翎 戚雪勇[1] 

机构地区：[1]江苏大学药学院,江苏镇江212013

出　　处：《江苏大学学报（医学版）》2015年第3期263-267,270,共6页Journal of Jiangsu University：Medicine Edition

基　　金：江苏省高等学校大学生实践创新训练计划项目(201410299029Z)

摘　　要：目的:建立表面增强拉曼光谱(surface enhanced Raman spectroscopy,SERS)法快速分析中药三七中的有效成分。方法:对三七药材进行简单的预处理,分别测试三七药材粉末、三七水提和醇提样品的表面增强拉曼光谱;再结合薄层色谱分离技术,测试三七醇提取样品中单一有效成分的表面增强拉曼光谱,并对所获得的图谱进行解析。结果:直接测试三七药材粉末、三七水提和醇提样品的表面增强拉曼光谱有微弱信号;三七醇提取样品经过TLC技术分离后的单一组分可获得较理想的表面增强拉曼光谱。结论:建立的快速分析三七有效成分的表面拉曼光谱法具有简便,快速,专属性好,灵敏度高等特点,可拓展到其他中药材的质量控制和分析鉴定中。Objective: To establish surface-enhanced Raman spectroscopy( SERS) method for rapid analysis of active ingredients in Panax notoginseng. Methods: Panax notoginseng was pretreated simply,then we tested the powder,water and alcohol extracts of Panax notoginseng by SERS respectively. We also got the spectrum of single active ingredient of the alcohol extracts combined with TLC,and analyzed these spectrum. Results: The powder,water and alcohol extracts of Panax notoginseng only could obtain weak signals of SERS directly,but the single active ingredient of Panax notoginseng separated by TLC technology could obtain its SERS spectra ideally. Conclusion: The method established in this article for rapid analysis of Panax notoginseng was efficient,rapid,specific,and high sensitivity,it can be extended to quality control and analysis in the identification of other herbal medicines.

关 键 词：表面增强拉曼光谱 三七 快速分析 

分 类 号：R284.1[医药卫生—中药学]