GITRL基因SNP-rs2236876分型方法的比较  

Comparison of method for GITRL SNP-rs2236876 genotyping

作　　者：吴晶晶[1] 仝佳[1] 陈娟[1] 袁靖[1] 田洁[1] 汤新逸[1] 王胜军[1] 

机构地区：[1]江苏大学医学院检验医学研究所,江苏镇江212013

出　　处：《江苏大学学报（医学版）》2015年第4期308-310,316,共4页Journal of Jiangsu University：Medicine Edition

基　　金：国家自然科学基金资助项目(81072453;31170849;30972748);江苏省自然科学基金资助项目(BK2011472);江苏省普通高校研究生科研创新计划项目(CXLX11_0608);江苏省卫生厅医学科研基金资助项目(Z201313);江苏省"青蓝工程"项目;江苏大学"拔尖人才工程"和高级人才基金项目

摘　　要：目的:对3种单核苷酸多态(single nucleotide polymorphism,SNP)分型方法进行比较,选择准确有效的SNP分型方法对GITRL SNP-rs2236876A/G进行基因分型。方法:采用Taqman-MGB探针法,限制性片段长度多态性聚合酶链式反应(cleaved amplification polymorphism sequence-tagged sites,PCR-RFLP)法和直接测序法对GITRL SNPrs2236876A/G进行分型并比较。结果:PCR-RLFP法分型结果直接通过电泳图可见,适合少量的实验对象;TaqmanMGB探针法通过不同的荧光区分不同的基因型,适合大量的实验对象;直接测序法交由测序公司测定,样本数量浮动范围较大,费用较高。结论:Taqman-MGB探针法适用于GITRL基因rs2236876A/G基因分型,PCR-RFLP法适用于基因分型结果的验证,直接测序法适用于鉴定有争议的PCR-RFLP法的验证结果。Objective: Comparing three single nucleotide polymorphism( SNP) genotyping methods,to choose an accurate and effective SNP genotyping method for rs2236876 A / G of GITRL gene. Methods: SNP genotyping for rs2236876 A / G of GITRL were performed and compared by Taqman-MGB probe,the PCRRLFP and direct sequencing. Results: PCR-RLFP showed the genotyping result by electrophoresis,which was suitable for a small number of experimental subjects. Taqman-MGB probe showed the genotyping result by fluorescence,which was suitable for a large number of experimental subjects. Direct sequencing method was operated by the sequencing company which was expensive. Conclusion: For rs2236876 A / G of GITRL gene,Taqman-MGB probe method could be chosen for genotyping,PCR-RFLP method confirmed the genotyping results and,at the same time,the direct sequencing finally validated the result of PCR-RFLP.

关 键 词：TAQMAN-MGB探针 单核苷酸多态分型 PCR-RFLP GITRL 

分 类 号：R440[医药卫生—诊断学]