萝卜硫素对免疫抑制小鼠的免疫调节作用  

Effects of Sulforaphane on Immunoregulation in Immunosuppression Mice

作　　者：高慧婕[1] 李春霞[2] 陈玉杰[1] 

机构地区：[1]济宁医学院药学院基础医学教研室,日照276826 [2]济宁医学院基础学院免疫学教研室,济宁272067

出　　处：《肿瘤防治研究》2015年第6期564-567,共4页Cancer Research on Prevention and Treatment

摘　　要：目的探讨萝卜硫素对免疫抑制小鼠免疫功能的保护作用。方法实验用小鼠随机分为4组:对照组、环磷酰胺(Cy)模型组、萝卜硫素高剂量组(30 mg/kg)和萝卜硫素低剂量组(15 mg/kg),灌胃给药,比较四组小鼠脾脏指数、胸腺指数、腹腔巨噬细胞吞噬功能、血清溶血素含量以及外周血淋巴细胞转化率;运用RT-PCR半定量法观察免疫调节基因TGF-β和PD-1m RNA的表达。结果与对照组相比,免疫抑制小鼠血清溶血素OD值(0.702±0.08)、外周血淋巴细胞的转化率(42.22%)、胸腺指数(1.25±0.21)和脾脏指数(2.73±0.26)、吞噬百分率(31.86%)和吞噬指数(0.73±0.06)显著下降(P<0.05);但是与环磷酰胺模型组比较,萝卜硫素组小鼠血清溶血素OD值(1.325±0.10)、外周血淋巴细胞的转化率(51.44%)、胸腺指数(1.89±0.31)和脾脏指数(3.80±0.46)、吞噬百分率(43.56%)和吞噬指数(1.15±0.08)明显增加(P<0.05),免疫调节基因TGF-β和PD-1m RNA的表达量下降,并且具有剂量依赖性。结论萝卜硫素可明显改善环磷酰胺免疫抑制小鼠的免疫功能,同时能有效抑制免疫调节基因TGF-β和PD-1的过度表达。Objective To investigate the immunoregulation effect of Sulforaphane(SF) in immunosuppression mice.Methods Institute of Cancer Research(ICR) mice were randomly divided into four groups:control group,Cyclophosphamide(Cy) model group,SF high-dose(30mg/kg) group and SF low-dose(15 mg/kg) group.The phagocytosis of macrophage,the content of serum hemolysin,lymphocyte transformation rate,spleen index and thymus index were analyzed.The expressions of TGF-β m RNA and PD-1 m RNA were detected by RT-PCR.Results Compared with control group,Cy-treated mice showed significant decrease in phagocytic percentage(31.86%) and index(0.73±0.06) of macrophage,hemolysin OD value(0.702±0.08),lymphocyte transformation rate(42.22%),spleen index(2.73±0.26),thymus index(1.25±0.21)(P<0.05).Compared with Cy model group,Sulforaphane increased the number of phagocytic percentage(43.56%) and index(1.15±0.08) of macrophage,hemolysin OD value(1.325±0.10),lymphocyte transformation rate(51.44%),spleen index(3.80±0.46),thymus index(1.89±0.31)(P<0.05) and down-regulated the expressions of TGF-β m RNA and PD-1 m RNA in a dose-dependent manner.Conclusion Sulforaphane could improve the immune function in immunodepression mice induced by Cy,and inhibit effectively the overexpression of TGF-β m RNA and PD-1 m RNA.

关 键 词：萝卜硫素 免疫调节 环磷酰胺 

分 类 号：R285.5[医药卫生—中药学]