左归降糖清肝方含药血清对脂肪酸孵育的HepG2细胞脂肪变性的影响  

Effect of Zuogui Jiangtang Qinggan Recipe on steatosis in Hep G2 cells induced by fatty acid

作　　者：成细华[1] 喻嵘[1] 胡伟 唐元[1] 罗文娟[1] 曾辛[1] 程莉娟[1] 

机构地区：[1]湖南中医药大学,湖南长沙410208 [2]湖南省工伤康复中心

出　　处：《中国老年学杂志》2015年第5期1307-1309,共3页Chinese Journal of Gerontology

基　　金：国家自然科学基金(81102593,81273753);地方高校国家级大学生创新创业训练计划(No.201210541003);中西医结合基础湖南省重点学科资助

摘　　要：目的探讨左归降糖清肝方含药血清对脂肪酸孵育的人肝细胞株(Hep G2)细胞内甘油三酯(TG)含量的影响。方法用混合脂肪酸孵育的Hep G 2细胞建立细胞脂肪变性模型;将实验分为正常组,模型组,5%、10%、15%含药血清组,油红O染色法检测细胞内脂质沉积,同时定量检测细胞内TG含量。结果 0.5 mmol/L混合脂肪酸孵育Hep G2细胞24 h,可诱导细胞内TG大量沉积,而10%、15%左归降糖清肝方含药血清可抑制脂肪酸这一效应,使细胞内TG含量明显下降(P<0.01)。结论左归降糖清肝方含药血清可改善脂肪酸诱导的Hep G2细胞脂肪变性。Objective To investigate the effect of Zuogui Jiangtang Qinggan Recipe( ZJQR) on triglyceride( TG) accumulation in Hep G2 cells induced by fatty acid mixed.Methods The cell model of steatosis was established by fatty acid mixed in Hep G2 cells.The experiments were divided into control,model,5%,10%,15% medicated serum groups.The amount of TG in Hep G2 cells was quantitatively analyzed by oil red O staining method.Results The fatty acid mixed( 0.5 mmol / L) resulted in significant steatosis in Hep G2 cells.The10%,15% medicated serum inhibited the effect of the fatty acid mixed,and significantly reduced the intracellular TG content.Conclusions ZJQR is able to ameliorate the excessive TG accumulation in Hep G2 cells induced by fatty acid mixed.

关 键 词：左归降糖清肝方 HEP G2细胞 脂肪变性 甘油三酯 

分 类 号：R285[医药卫生—中药学]