扎里奴思方对局灶性脑缺血再灌注损伤大鼠P-糖蛋白的影响  

Effects of Zhalinusi Fang on the P-glycoprotein changes after focal cerebral ischemia-reperfusion in rats

作　　者：李娟[1] 吴鹏[2] 刘敬霞[1] 刘洋[1] 

机构地区：[1]宁夏医科大学附属国医中医医院,宁夏银川750004 [2]固原市人民医院

出　　处：《中国老年学杂志》2015年第5期1319-1321,共3页Chinese Journal of Gerontology

基　　金：国家自然科学基金(No.81260569);宁夏科技支撑计划(No.2012ZYH230)

摘　　要：目的比较缺血损伤不同时段大鼠血脑屏障P-糖蛋白(P-gp)时征表达及回药扎里奴思方对其表达的影响。方法 SD大鼠随机分为假手术组、模型组、尼莫地平组、扎里奴思方组,线栓法制备脑缺血再灌注模型,分别灌胃给药,于缺血再灌注后1、3、7、14 d取脑,检测大鼠脑皮质及海马区P-gp的表达。结果免疫组织化学染色结果显示,假手术组与模型组大鼠脑皮质及海马缺血区均可见P-gp阳性染色。与假手术组比较,不同再灌注时间模型组P-gp表达量均有显著性差异(P<0.01),皮质及海马缺血区P-gp表达量均升高;与模型组比较,扎里奴思方组及尼莫地平组大鼠脑皮质及海马区P-gp表达量明显降低,尤以扎里奴思方3、14 d组为明显(P<0.01)。结论扎里奴思方发挥抗脑缺血损伤、神经元保护作用机制可能是通过调节血脑屏障上P-gp的双向表达而实现的。Objective To research the P-glycoprotein( P-gp) expression on the blood-brain barrier at different times and the effect of Zhalinusi Fang after focal cerebral ischemia-reperfusion( I / R) in rats.Methods SD rats were divided randomly into sham-operated,model,nimodipine and Zhalinusi Fang groups.Rat focal cerebral I / R model was established by middle cerebral artery occlusion with nylon thread.After rats were administered orally with corresponding drugs 1,3,7,14 d,the brain was taken out.Immunohistochemical method was used to detect P-gp expression in cerebral cortex and hippocampus.Results Immunohistochemical staining showed that in sham-operated and model groups,P-gp positive staining was visible at ischemic cerebral cortex and hippocampus region.Compared with that of sham-operated group,P-gp expressions of different reperfusion time model groups were significant different( P < 0.01),P-gp expression at ischemic cortex and hippocampus were increased.Compared with those of model group,of P-gp expression at cerebral cortex and hippocampus of ZLNSF and nimodipine groups were significantly decreased( P < 0.01).Conclusions Zhalinusi Fang could promote the cerebral protection,its mechanism may be related to bidirectional regulation the expression of P-gp at blood-brain barrier.

关 键 词：扎里奴思方 脑缺血再灌注损伤 血脑屏障 P-糖蛋白 

分 类 号：R29[医药卫生—民族医学]