丁苯酞对慢性酒精中毒大鼠海马内硫化氢和纤维状肌动蛋白的影响  

The effect of Butylphthalide on H2S and F-actin in hippocampus of chronic alcoholism rats

作　　者：徐磊[1,2] 姜洪波[1,3] 安娜[1,2] 董献红 杜爱林[1,2] 张瑞岭[1,2,4] 

机构地区：[1]新乡医学院第二附属医院,河南新乡453003 [2]新乡医学院 [3]新乡医学院第三附属医院 [4]河南省生物精神病学重点实验室

出　　处：《中国老年学杂志》2015年第8期2161-2163,共3页Chinese Journal of Gerontology

基　　金：国家自然科学基金(81471351);河南省教育厅课题(15A310006);河南省卫生科技创新型人才工程专项(3052)

摘　　要：目的探究丁苯酞(NBP)对慢性酒精中毒大鼠学习记忆能力、海马内硫化氢(H2S)和纤维状肌动蛋白(F-actin)含量的影响。方法将50只SD雄性大鼠随机分为5组,除对照组外,将含酒的水作为大鼠唯一水来源,并逐渐增加酒精含量,采用自由饮用方法建立慢性酒精中毒模型。42 d后按慢性酒精中毒大鼠戒断评分表于末次饮酒后6 h检测大鼠模型,NBP低、中、高剂量组大鼠灌胃不同剂量NBP(大约14 d),饮酒结束后利用Y型电迷宫测试酒精中毒大鼠的学习记忆能力,随后检测海马组织H2S和F-actin的含量。结果与对照组大鼠学习记忆成绩(40.38±5.97)、H2S含量(23.25±3.61)和F-actin在阳性细胞中的表达相比,模型组大鼠学习记忆成绩(83.25±5.47)和H2S含量(43.86±6.95)均升高,F-actin的阳性表达降低(P<0.01)。与模型组大鼠相比,NBP中、高剂量组大鼠学习记忆成绩(64.75±4.71)、(70.00±4.72)和H2S含量(32.21±5.32)、(36.78±7.29)nmol/g均降低,F-actin的阳性表达升高(P<0.01)。结论 NBP能够减轻慢性酒精中毒大鼠对酒精的依赖,缓解酒精对大鼠学习记忆能力的影响,可能与NBP影响H2S和F-actin的含量有关。Objective To investigate the effect of butylphthalide( NBP) on the learning and memory abilities of chronic alcoholism rats,H2 S content and the expression of F-actin in the hippocampus of chronic alcoholism rats.Methods 50 male SD rats were randomly divided into five groups.in addition to control group,the rats were reared by the water containing wine. The alcohol content was gradually increased to establish the free drinking modeling. After 42 d,6 h after drinking rat models were detected by chronic alcoholism withdrawal score sheet.The low,medium and high dose NBP group rats were fed different doses of NBP,the learning and memory were tested bya Y-shaped maze detection the H2 S content of the hippocampus and the expression of F-actin were measured.Results Compared to learning and memory scores( 40.38±5.97),H2 S content( 23. 25 ± 3. 61) and the expression of F-actin in control group rats,learning and memory scores( 83. 25 ±5. 47) and H2 S content( 43. 86±6. 95) in rats of model group were increased and F-actin positive expression was reduced( P<0.01). Compared with those of model group,learning and memory scores( 64. 75 ± 4. 71)( 70. 00 ± 4. 72) and H2 S content( 32. 21 ± 5. 32)( 36. 78 ±7. 29) in medium,high dose NBP group were reduced and F-actin positive expression was increased( P< 0.05). Conclusions NBP could reduce of chronic alcoholism rat alcohol dependence,and mitigate the impact of alcohol on learning and memory abilities of rats with NBP might affect H2 S and F-actin contents relevant.

关 键 词：丁苯酞 慢性酒精中毒 纤维状肌动蛋白 硫化氢 学习记忆能力 

分 类 号：R965[医药卫生—药理学]