香蕉胚性愈伤组织的诱导及胚性细胞悬浮系的建立  

The induction of embryogenic callus and the establishment of embryogenic cell suspension of Musa spp.

作　　者：徐春香[1] B.PANIS H.STROSSE R.SWENNEN 李华平[1] 肖火根[1] 范怀忠[1] 

机构地区：[1]华南农业大学植物病毒研究室,广东广州510642 [2]比利时荷语鲁汶大学热带作物改良实验室

出　　处：《华南农业大学学报》2004年第1期70-73,共4页Journal of South China Agricultural University

基　　金：国家自然科学基金资助项目(39870434);广东省自然科学基金资助项目(990714);广东省科技攻关项目(C20209)

摘　　要：分别以3个香蕉品种的多芽体茎尖和5个香蕉品种的未成熟雄花为外植体,诱导产生胚性愈伤组织.结果分别从2个基因组类型为AAA的品种的多芽体茎尖和未成熟雄花获得了胚性愈伤组织,而未能从基因组类型为AAB或ABB的香蕉品种中获得胚性愈伤组织.胚性愈伤组织的诱导率受基因组类型、品种和培养条件等因素的影响.以上述4个品种的胚性愈伤组织为起始材料成功建立了胚性细胞悬浮系,不同香蕉品种,从其胚性愈伤组织建立胚性细胞悬浮系的难易程度有所不同.Embryogenic callus was successfully induced starting from immature male flowers in 2 out of 5 banana cultivars and starting from scalps in 2 out of 3 cultivars. All these 4 cultivars from which embryogenic callus could be induced belonged to Musa AAA group. The frequency of embryogenic callus induction was depended on genotypes, cultivars and incubation conditions etc. Embryogenic cell suspensions (ECSs) were initiated successfully from embryogenic callus of all these 4 cultivars. The possibility of getting ECSs from embryogenic callus was also cultivar-dependent.

关 键 词：香蕉 胚性愈伤组织 基因诱导 胚性细胞 悬浮系统 

分 类 号：S668.1[农业科学—果树学]