微流控芯片停流液-液萃取技术的研究  

Stopped-flow Liquid-liquid Extraction on Microfluidic Chips

作　　者：方群[1] 陈宏[1] 蔡增轩[1] 

机构地区：[1]浙江大学化学系微分析系统研究所,杭州310028

出　　处：《高等学校化学学报》2004年第2期261-263,共3页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金重大项目 (批准号 :2 0 2 990 3 0 );国家"八六三"计划项目 (批准号 :2 0 0 2 AA2 Z2 0 42 ;2 0 0 2 AA40 42 40 );教育部科学技术研究重点项目 (批准号 :0 10 93 )资助

摘　　要：In this work, two novel approaches were developed for miniaturized liquid-liquid(L-L) extraction on microfluidic chips, based on a stopped-flow extraction technique. In the first approach, trapped droplets extraction mode, organic solvent droplets of a few hundred 10 -12 L were trapped within micro recesses fabricated in the channel walls of microfluidic chips, and analytes in aqueous streams flowing over the droplets were transferred into them, affecting a preconcentration. In the second approach, a stable interface between stationary organic phase and continuously flowed aqueous phase was formed by stopping the flow of organic phase. Analytes were transferred from the aqueous phase into the organic phase on the interface. Enrichment factors exceeding 1 000 and 300 were achieved with a preconcentration period of 20 min with sample consumption lower than 10 μL for trapped droplets and stopped-flow microextraction. In situ laser induced fluorescence detection of the concentrated analyte was performed following the preconcentration.In this work, two novel approaches were developed for miniaturized liquid-liquid(L-L) extraction on microfluidic chips, based on a stopped-flow extraction technique. In the first approach, trapped droplets extraction mode, organic solvent droplets of a few hundred 10 -12 L were trapped within micro recesses fabricated in the channel walls of microfluidic chips, and analytes in aqueous streams flowing over the droplets were transferred into them, affecting a preconcentration. In the second approach, a stable interface between stationary organic phase and continuously flowed aqueous phase was formed by stopping the flow of organic phase. Analytes were transferred from the aqueous phase into the organic phase on the interface. Enrichment factors exceeding 1 000 and 300 were achieved with a preconcentration period of 20 min with sample consumption lower than 10 μL for trapped droplets and stopped-flow microextraction. In situ laser induced fluorescence detection of the concentrated analyte was performed following the preconcentration.

关 键 词：微流控芯片 液-液萃取技术 停流技术 芯片捕陷液滴萃取系统 芯片停流萃取系统 

分 类 号：O657[理学—分析化学]