应用停流时间分辨光谱研究氧合血红蛋白与过氧化氢反应中间体的动力学和机理  

Studies on the Kinetics and Mechanism of Intermediates of Rexaction between Oxyhemoglobin and H_2O_2 Using Stopped-flow Time-resolved Spectrophotometry

作　　者：王瑞勇[1] 蔡汝秀[1] 林智信[1] 

机构地区：[1]武汉大学化学与分子科学学院,武汉430072

出　　处：《高等学校化学学报》2004年第2期267-269,共3页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金 (批准号 :2 0 2 75 0 2 7)资助

摘　　要：Interaction between oxyhemoglobin and H2O2 was studied by the aid of stopped-flow time-resolved spectrophotometry. The consecutive reaction kinetics of oxyhemoglobin with H2O2 suggested the presence of an intermediate species. The influence of pH, concentration of H2O2, and temperature on the pseudo-first order rate constants was studied. The kinetic experiments performed at different temperatures allowed to calculate the activation energy. A reasonable mechanism was also proposed.Interaction between oxyhemoglobin and H_2O_2 was studied by the aid of stopped-flow time-resolved spectrophotometry. The consecutive reaction kinetics of oxyhemoglobin with H_2O_2 suggested the presence of an intermediate species. The influence of pH, concentration of H_2O_2, and temperature on the pseudo-first order rate constants was studied. The kinetic experiments performed at different temperatures allowed to calculate the activation energy. A reasonable mechanism was also proposed.

关 键 词：氧合血红蛋白 过氧化氢 相互作用 反应动力学 反应机理 停流时间分辨光谱 

分 类 号：Q513[生物学—生物化学] Q592.1