番茄子叶外植体转化系统的优化  

To Optimize the Factors that Affected Transformation System of Tomato Cotyledon Explant

作　　者：江晓玲[1] 俞守义[1] 贺竹梅[2] 彭志强[1] 祁瑜[2] 

机构地区：[1]第一军医大学热带军队卫生学系流行病学教研室,广东广州510515 [2]中山大学生命科学院生物工程中心,广东广州510275

出　　处：《云南植物研究》2004年第1期118-120,共3页Acta Botanica Yunnanica

基　　金：广东省自然科学基金重点项目 ( 0 130 75)

摘　　要：目的 :对番茄子叶外植体的转化进行优化 ,为进行转基因番茄的研究奠定基础。方法 :通过正交设计实验 ,以子叶外植体的存活天数为观察指标 ,分别对农杆菌稀释浓度、浸染时间和转化方式等条件进行优化。结果 :在子叶预培养 2d、活化农杆菌稀释倍数约 30倍、侵染时间为 1 0min、共培养时间为 2d的情况下 ,子叶存活时间最长 ,抗性芽产生频率达 2 5 %。结论 :获得了高效的番茄子叶外植体转化系统 。Aim:To optimize the factors that affect transformation system of tomato cotyledon explant.Method:By orthogonal design of statistics,factors that affect transformation such as the Agrobacterium concentration,infiltration time and transform style were assembled.Result:Best transformation system could be set up by the contidition of 2 days pre-culture,30-fold-dilution,10-minute-infiltation of Agrobacterium solution,and 2 days co-cultivation.The frenquency of success transformation reached to 25%,higher than average level at present.Conclusion:Factors that affect transformation system of tomato cotyledon explant were successfully optimized.

关 键 词：番茄 子叶外植体 转化系统 农杆菌 

分 类 号：S641.2[农业科学—蔬菜学]