丹参酮ⅡA对心肌肥厚的作用及其机制研究  

Effect of Salvia Miltiorrhiza Bge on cardiac hypertrophy and its mechanism

作　　者：龚丽娅[1] 郑智[1] 黄俊[1] 陈钧[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院急诊科,湖北武汉430030

出　　处：《中国急救医学》2004年第2期116-118,共3页Chinese Journal of Critical Care Medicine

基　　金：湖北省科技攻关项目资助 (No .2 0 0 2AA3 0 1C2 9)

摘　　要：目的　在原代培养的新生大鼠心肌细胞上 ,观察丹参酮ⅡA(TanshinoneⅡA ,TSN)对血管紧张素Ⅱ (AngiotensinⅡ ,AngⅡ )诱导的心肌细胞肥大的影响。方法　采用相差显微镜测量细胞大小 ,3 H -亮氨酸掺入法测定心肌细胞蛋白质合成速率作为心肌细胞肥大的指标 ;用逆转录聚合酶链式反应 (RT -PCR)检测心肌细胞原癌基因c -fosmRNA的表达。结果　TSN能抑制AngⅡ诱导的心肌细胞增大 ,蛋白质合成速率的显著增加 (P <0 0 5 )及心肌细胞原癌基因c -fosmRNA表达的增强 (P <0 0 5 ) ,其效果与AngⅡ受体阻滞剂缬沙坦 (Valsartan ,Val)相似。结论　TSN可以抑制AngⅡ诱导的心肌细胞肥大 ,这与其抑制了原癌基因c -fos的表达有关。Objective To investigate the effect of Tanshinone Ⅱ A(TSN) on the hypertrophy induced by angiotensin Ⅱ(AngⅡ)in the primary culture of neonatal rat cardiomyocytes.Methods As the index of cardiomyocyte hypertrophy,the cell size was determined by phase contrast microscope and protein synthesis rate was measured by 3 H-Leucine incorporation.The proto-oncogene c-fos mRNA expression was assessed using reverse transcription polymerase chain reaction(RT-PCR).Results The cell size and protein synthesis rate and proto-oncogene c-fos mRNA expression of cardiomyocytes increased significantly after AngⅡ treatment,and TSN inhibited these effect of Ang Ⅱ.Conclusion TSN can prevent the hypertrophy of cardiomyocyte induced by AngⅡ,which may relate to the decreased expression of proto-oncogene c-fos mRNA.

关 键 词：血管紧张素Ⅱ 丹参酮ⅡA 心肌细胞肥大 C-FOS 

分 类 号：R285.5[医药卫生—中药学]