流动注射抑制化学发光法测定痕量强力霉素  

Flow Injection Analysis of Trace Amounts of Doxycycline with Inhibited Chemiluminescence Detection

作　　者：李念兵[1] 段建平[2] 陈红青[2] 陈国南[1] 

机构地区：[1]福州大学化学化工学院,福建福州350002 [2]福州大学分析测试中心,福建福州350002

出　　处：《光谱学与光谱分析》2004年第1期15-17,共3页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金 (2 0 1 750 0 5);福州大学博士后基金;福州大学科学发展基金资助项目

摘　　要：基于在NaOH介质中 ,强力霉素对Luminol KMnO4 体系发光反应具有强烈的抑制作用 ,建立起流动注射抑制化学发光测定痕量强力霉素的新方法。强力霉素在 0 0 0 5～ 5 0 μg·mL- 1 浓度范围内 ,采用不同的KMnO4 溶液浓度 ,分段建立起抑制化学发光强度与其浓度间良好的线性关系 ,方法的检出限为 2 0×10 - 3μg·mL- 1 。A novel flow injection chemiluminescence method has been developed for the determination of doxycycline(DC) based on the inhibition of chemiluminescence reaction of Luminol-KMnO 4 by using DC in sodium hydroxide medium. Using different concentrations of KMnO 4, a series of good linear regression equations of decreased chemiluminescence intensity and the concentration of DC were obtained with different subsections in the concentration range of 0.005-5.0 μg·mL -1. The detection limit (3σ) is 2.0×10 -3μg·mL -1. The method has been successfully applied to the determination of DC in a pharmaceutical preparation.

关 键 词：强力霉素 痕量测定 含量测定 流动注射 抑制化学发光法 四环素类抗生素 

分 类 号：R978.14[医药卫生—药品] R927.2[医药卫生—药学]