褶合光谱法考察羟自由基清除剂对紫外照射致DNA变异的作用  

Convolution Spectrometric Determination of the Effect of Hydroxyl Radical Scavenger on UV Radiation-induced DNA Aberrance

作　　者：陆峰[1] 肖杰[1] 林培英[1] 杨根金[1] 

机构地区：[1]第二军医大学药学院,上海200433

出　　处：《光谱学与光谱分析》2004年第1期82-84,共3页Spectroscopy and Spectral Analysis

基　　金：国家自然科学基金 (30 1 0 0 2 33)资助项目

摘　　要：采用褶合光谱法考察了紫外线UV A ,UV B ,UV C导致的DNA变异 ,并以褶合光谱差谱值的形式量化表达DNA细微突变的程度。DNA受UV A ,UV B ,UV C作用后 ,差谱值依次增大 ,表明变异程度增大 ;添加二甲亚砜、甘露醇等羟自由基清除剂以后 ,在UV A ,UV B ,UV C作用下 ,差谱值有不同程度的降低 ,呈现一定的抑制变异作用。尽管DNA的变异程度有所区别 ,但它们的差谱点分布区域相似 ,提示UV A ,UV B ,UV C导致的DNA变异类型可能相似 。Convolution spectrometry was applied to determining the DNA aberrance induced by UV-A, UV-B and UV-C radiation, and the extent of minute aberrance was quantitatively expressed by the differential value of convolution spectra. The differential values of DNA solutions irradiated by UV-A, UV-B and UV-C increase in turn, indicating an increscent DNA aberrance. The addition of dimethylsulfoxide or mannitol to DNA solution decreases the differential value in respective way under UV-A, UV-B and UV-C radiation, representing different inhibitory effects. Dimethylsulfoxide decreases the UV-A-induced DNA aberrance by 60%(approximate value), UV-C-induced aberrance by 35%(approximate value), and UV-B-induced aberrance by 20%(approximate value), while mannitol shows similar inhibitory effects (40%, approximate value) for UV-A, UV-B and UV-C -induced DNA aberrance. Although the extent of DNA aberrance varies, the distribution of differential points of convolution spectra reveals that the types of UV radiation-induced DNA aberrance are similar, and the inhibitory mechanism of two hydroxyl radical scavengers is possibly the same.

关 键 词：羟自由基清除剂 紫外线照射 DNA变异 褶合光谱法 

分 类 号：Q523[生物学—生物化学] Q503