川西獐牙菜与抱茎獐牙菜中酮的高效液相色谱分析  

Determination of Xanthones in Swertia mussotii and Swertia franchetiana by High Performance Liquid Chromatography

作　　者：宋娅莉[1] 胡凤祖[1] 林鹏程[2] 卢永昌[2] 师治贤[1] 

机构地区：[1]中国科学院西北高原生物研究所 [2]青海民族学院化学系,青海西宁810007

出　　处：《色谱》2004年第1期51-53,共3页Chinese Journal of Chromatography

摘　　要：采用反相高效液相色谱法同时分离和测定了龙胆科獐牙菜属的川西獐牙菜和抱茎獐牙菜中几种酮的含量。色谱柱为KromasilC18(250mm×4 60mmi d ,5μm),流动相为甲醇 0 1%的磷酸水溶液(体积比为73∶27),流速1mL/min,检测波长260nm,柱温20℃。实验结果表明,该方法具有很好的线性关系与精密度,方法简单、准确、实用性强。A simple and rapid method for simultaneous separation and determination of xanthones in Swertia mussotii and Swertia franchetiana by high performance liquid chromatography has been established. The analysis was performed on a Kromasil C_18column (250 mm×4.60 mm i.d., 5 μm) (at 20 ℃) eluted with methanol and 0.1% aqueous phosphoric acid (73∶27 in volume ratio) as mobile phase at a flow rate of 1.0 mL/min and with UV detection at 260 nm. Results showed that xanthones were separated successfully from each other and from other interfering components. There was a good linear relationship between the content of component and its peak area for each xanthone, with the correlation coefficients of 0.999 2-0.999 9. The convenient method can be used for quantitative analysis of xanthones.

关 键 词：中草药 川西獐牙菜 抱茎獐牙菜 SAN酮 高效液相色谱分析 分离 含量测定 

分 类 号：R284.2[医药卫生—中药学]