血管组织工程基质材料及管形支架的制备  

Preparation of matrix material and tubular scaffold for blood vessel tissue engineering

作　　者：熊猛[1] 鲁开化[1] 商庆新[2] 曹谊林[2] 毛津淑[3] 杨洪义[4] 夏文森[1] 

机构地区：[1]第四军医大学西京医院整形外科中心,陕西西安710032 [2]上海组织工程研究与开发中心 [3]天津大学高分子材料研究所 [4]协和医科大学生物医学工程研究所

出　　处：《西北国防医学杂志》2004年第1期3-6,共4页Medical Journal of National Defending Forces in Northwest China

基　　金：国家自然科学基金资助项目 ( 30 0 70 777)

摘　　要：目的 :几种血管基质材料的制作及比较。方法 :采用EDC交联胶原和粘多糖材料 ;采用戊二醛和热交联得到明胶加壳聚糖材料 ;应用去垢剂 ,渗透压改变和核酸酶消化的方法 ,对猪主动脉进行去细胞处理 ,得到完整的去细胞纤维支架。采用直接模具成型 ,形成多种管形支架。结果 :得到明胶加壳聚糖、完整的猪主动脉去细胞纤维支架等几种基质材料 ;管形支架外径 1cm ,内径 0 .6～ 0 .8cm ,放置培养液中 10d ,可保持管腔形状 ,不塌陷 ,不变形。结论 :明胶加壳聚糖三维结构好 ,主动脉脱细胞基质仍显致密 ,采用模具直接成型、冷冻干燥技术相结合 。Objective: To prepare and compare different matrix mat er ials for blood vessel tissue engineering. Methods: T he spongy collagen/chondroitin sulfate scaffold was crosslinked with EDC; the co llagen/chitosan scaffold was crosslinked with glutaraldehyde and heat; Porcine acellular aorta were obtained through a cellula r extraction procedure. Tubular porous vascular scaffolds were made under vacuum freeze condition in a special annular model.Results: The tubular porous scaffolds have elasticity, stubbornness and hydrophilic, can maintain their shap es in culture medium for long time. Conclusion: Several matrix materials for vessels as tubular scaffo ld have been obtained; it could be used for further studies.

关 键 词：管型支架 基质材料 血管组织工程 

分 类 号：R318.08[医药卫生—生物医学工程]