胡芦巴中皂苷成分的研究 Ⅱ.新皂苷B和C的分离纯化及化学结构测定  

Studies on saponins from seeds of Trigonella foenum-graecum Ⅱ.Isolation and structural elucidation for two new saponins B and C

作　　者：徐学民[1] 王笳[1] 杨红[1] 黄卫平[1] 袁崇军[1] 

机构地区：[1]四川省中药研究所,四川成都610041

出　　处：《中草药》2004年第2期127-130,共4页Chinese Traditional and Herbal Drugs

摘　　要：目的　对胡芦巴 Trigonella foenum- graecum种子所含的皂苷成分作深入研究。方法　采用硅胶 H柱色谱 ,干柱色谱等方法从总皂苷中分离纯化皂苷成分并通过 1 3C- NMR,FAB- MS,DEPT等波谱法及部分水解获得次生苷的方法测定了化学结构。结果　从总皂苷中分出两个新皂苷 B和 C,均为薯蓣皂苷元的四糖苷。经测定皂苷 B的化学结构为 :薯蓣皂苷元 - 3- O-α- L -鼠李吡喃糖 (1→ 3) -α- L -鼠李吡喃糖 (1→ 4 ) -β- D -葡萄吡喃糖 (1→ 4 ) -β- D-葡萄吡喃糖苷 ;皂苷 C的结构为薯蓣皂苷元 - 3- O- β- D-葡萄吡喃吡喃糖 (1→ 4 ) - α- L-鼠李吡喃糖 (1→ 4 ) - β- D-葡萄吡喃糖(1→ 4 ) -β- D-葡萄吡喃糖苷。结论　皂苷 B和Object To do detail investigation of the saponins from the Chinese materia medica Huluba (the seeds of Trigonella foenum graecum L ) Methods The pure saponins from the total saponins were isolated by employing the column chromatography and dry column chromatography of silica gel H Their chemical structures were elucidated by 13 C NMR , FAB MS, DEPT spectroscopic evidence and the results of the fraction hydrolysis of acquiring their secondary glucosides were obtained Results Two new saponins B and C were isolated and both were the glucosides consisted by four molecules of sugar with diosgenin The chemical structure of B is: diosgenin 3 O α L rhamnopyranosyl (1→3) α L rhamnopyranosyl (1→4) β D glucopyranosyl (1→4) β D glucopyranoside And saponin C is: diosgenin 3 O β D glucopyranosyl (1→4) α L rhamnopyranosyl (1→4) β D glucopyranosyl (1→4) β D glucopyranoside Conclusion Saponins B and C are two new ones with four molecules of sugar respectively

关 键 词：胡芦巴 皂苷B 皂苷C 

分 类 号：R284.1[医药卫生—中药学]