HPLC测定龙血竭中对羟基苯甲酸乙酯的含量  

HPLC determination of ethyl p-hydroxybenzoate in Dragon's Blood

作　　者：张庆云[1] 崔兰贵[1] 胡迎庆[1] 

机构地区：[1]中国人民武装警察部队医学院,天津300162

出　　处：《中草药》2004年第2期197-199,共3页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目 ( 3 0 0 70 0 88)

摘　　要：目的　建立测定龙血竭中对羟基苯甲酸乙酯含量的方法。方法　以 C1 8反相键合硅胶为固定相 ,乙腈 - 1%冰醋酸 (31∶ 6 9)为流动相 ,检测波长为 2 5 7nm,用外标法定量。结果　对羟基苯甲酸乙酯在 0 .2 0 6～ 2 .884 ng有良好线性关系 ,r=0 .9998,平均回收率为 96 .0 2 % ,RSD为 1.87%。结论　本方法是测定龙血竭中对羟基苯甲酸乙酯含量的快速、简便。Object To establish a HPLC method for determination of ethyl p hydroxybenzoate in Dragon's Blood. Methods Using C 18 reverse phase column and acetonitrile 1% acetic acid (31∶69) as mobile phase, detecting at 257 nm and quantitating with external standard method. Results The standard curves of ethyl p hydroxybenzoate showed good linearity over the range of 0.206-2.884 ng, r=0.999 8. The average recovery was 96.02% and RSD was 1.87%. Conclusion The method is quick and simple, and it is accurate and reliable for the determination of ethyl p hydroxybenzoate in Dragon's Blood.

关 键 词：HPLC 龙血竭 对羟基苯甲酸乙酯 

分 类 号：R282.6[医药卫生—中药学]