双黄补缓释药条对实验性牙周组织再生中BMP2和IGF1表达的影响  

Expression of BMP2 and IGF1 in the periodontium of experimental regeneration a ffected by slow-releasing Shuanghuangbu strip

作　　者：许彦枝[1] 王士杰[1] 张东妹[1] 王永军[1] 

机构地区：[1]河北医科大学第四医院口腔科,石家庄050011

出　　处：《实用口腔医学杂志》2004年第1期47-51,共5页Journal of Practical Stomatology

基　　金：河北省科技厅资助课题(0 2 2 76 1 0 3D -19)

摘　　要：目的 :探讨双黄补缓释药条对实验性牙周组织再生过程中骨形成蛋白 2 (BMP2 )和胰岛素样生长因子 1(IGF1)的表达及牙周组织再生修复的影响。方法 :以 4只beagle狗双侧上下颌第二切牙及尖牙作为手术部位 ,共 3 2个 ,将其配对分组为实验组和对照组各 16个牙位。实验部位造成人工牙周骨缺损 ,应用双黄补缓释药条 ;对照组为单纯翻瓣术。每只狗选择 2个牙位作为正常组 ,不做任何处理。分别于术后 1个月和 3个月取标本 ,应用SP法进行免疫组织化学染色 ,定量分析牙周组织中BMP2和IGF1的表达。结果 :实验组术后 1个月和 3个月BMP2和IGF1阳性率显著高于对照组同时期相应值 (P <0 .0 5)。结论 :双黄补缓释药条能增加实验动物BMP2和IGF1在牙周组织中的表达 ,刺激牙周膜细胞向化生性骨细胞分化 。Objective:To investigate the effect of Shuanghuangbu slow releasing strip (SRSS) on the expression of BMP2 and IGF1 in the experimental regeneration of periodontium. Methods:Periodontal defects were surgically made around the second incisor and the ca nine in four beagle dogs .32 defects were randomly divided into experimental gr oup and control group with 16 defects in each group. SRSS was applied to the e xperimental group and no disposition to periodontal defect in the control group. Two teeth of each dog were randomly chosen as the natural controls.Specimens we re respectively obtained one month and three months after operation. BMP2 and I GF1 were quantitatively analyzed by immunohistochemistry. Results:The positive rate of BMP2 and IGF1 in experimental group were higher t han those in the control( P< 0.05) 1 month and 3 months after operation. Conclusion:SRSS can increase the expression of BMP2 and IGF1 in the periodontium.

关 键 词：双黄补缓释药条 实验 牙周组织再生 BMP2 IGF1 骨形成蛋白2 胰岛素样生长因子1 中药 牙周骨缺损 

分 类 号：R285[医药卫生—中药学]